Pep4c 

Pep4c is an inactive control peptide for Pep2m (HY-P1058). Pep4c lacks functional activity to disrupt Protein Interacting with C Kinase 1 (PICK1)-AMPA receptor (GluA2) or N-ethylmaleimide-sensitive factor (NSF)-GluA2 interactions. Pep4c is used as a negative control in experiments to validate the specificity of Pep2m's effects on AMPA receptor trafficking and synaptic plasticity^{[1][2][3][4][5][6]}.

Pep4c does not reduce the current response to topically applied AMPA, nor does it affect the frequency and amplitude of AMPA-mEPSCs in cultured hippocampal neurons^[1].

Pep4c does not alter the surface expression of AMPA receptors in cultured hippocampal neurons but improves the colocalization of GluR2 and synaptophysin in cultured hippocampal neurons^[1].

Pep4c does not affect long-term depression (LTD) and basal parallel fiber excitatory postsynaptic current (PF-EPSC) in hippocampal slices, but can induce long-term potentiation (LTP) by 1 Hz parallel fiber (PF) stimulation^[2][3].

Pep4c does not bind to NSF and does not alter autistic EPSCs evoked in Pep2m-loaded neurons^[4].

Pep4c does not alter the release of radiolabeled D-aspartate ([³H]D-ASP) in rat hippocampal synaptosomes, either in the absence (not shown) or in the presence of 50 μM (S)AMPA^[5].

Pep4c does not affect tritium release induced by 10 μM NMDA/1 μM Glycine (HY-Y0966), but caused changes in [³H]D-ASP release induced by 10 μM NMDA/1 μM Glycine and 10 μM NMDA/1 μM Glycine/50 μM (S)AMPA^[5].

Pep4c does not attenuate synaptic transmission^[6].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

1146.41

C₄₈H₉₁N₁₇O₁₃S

243843-43-8

Lys-Arg-Met-Lys-Val-Ala-Lys-Ser-Ala-Gln

KRMKVAKSAQ

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Noel J, et al. Surface expression of AMPA receptors in hippocampal neurons is regulated by an NSF-dependent mechanism. Neuron. 1999 Jun;23(2):365-76.  [Content Brief]

  [2]. Lüthi A, et al. Hippocampal LTD expression involves a pool of AMPARs regulated by the NSF-GluR2 interaction. Neuron. 1999 Oct;24(2):389-99.  [Content Brief]

  [3]. Gallimore AR, et al. Switching On Depression and Potentiation in the Cerebellum. Cell Rep. 2018 Jan 16;22(3):722-733.  [Content Brief]

  [4]. Cingolani LA, et al. Activity-dependent regulation of synaptic AMPA receptor composition and abundance by beta3 integrins. Neuron. 2008 Jun 12;58(5):749-62.  [Content Brief]

  [5]. Summa M, et al. Hippocampal AMPA autoreceptors positively coupled to NMDA autoreceptors traffic in a constitutive manner and undergo adaptative changes following enriched environment training. Neuropharmacology. 2011 Dec;61(8):1282-90.  [Content Brief]

  [6]. Correia SS, et al. Motor protein-dependent transport of AMPA receptors into spines during long-term potentiation. Nat Neurosci. 2008 Apr;11(4):457-66.  [Content Brief]