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  4. p-Nitrophenyl α-L-fucoside

p-Nitrophenyl α-L-fucoside  (Synonyms: PNP α-L-Fuc)

Cat. No.: HY-134420 Purity: 98.51%
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p-Nitrophenyl α-L-fucoside (PNP α-L-Fuc) is a substrate for the determination of α-L-fucosidase.

For research use only. We do not sell to patients.

p-Nitrophenyl α-L-fucoside Chemical Structure

p-Nitrophenyl α-L-fucoside Chemical Structure

CAS No. : 10231-84-2

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Based on 1 publication(s) in Google Scholar

p-Nitrophenyl α-L-fucoside Related Antibodies

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Description

p-Nitrophenyl α-L-fucoside (PNP α-L-Fuc) is a substrate for the determination of α-L-fucosidase[1].

Molecular Weight

285.25

Formula

C12H15NO7
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

O[C@@H]([C@@H]([C@@H]([C@H](C)O1)O)O)[C@@H]1OC2=CC=C([N+]([O-])=O)C=C2
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 25 mg/mL (87.64 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H2O : 4 mg/mL (14.02 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.5057 mL 17.5285 mL 35.0570 mL
5 mM 0.7011 mL 3.5057 mL 7.0114 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O / DMSO 1 mM 3.5057 mL 17.5285 mL 35.0570 mL 87.6424 mL
5 mM 0.7011 mL 3.5057 mL 7.0114 mL 17.5285 mL
10 mM 0.3506 mL 1.7528 mL 3.5057 mL 8.7642 mL
DMSO 15 mM 0.2337 mL 1.1686 mL 2.3371 mL 5.8428 mL
20 mM 0.1753 mL 0.8764 mL 1.7528 mL 4.3821 mL
25 mM 0.1402 mL 0.7011 mL 1.4023 mL 3.5057 mL
30 mM 0.1169 mL 0.5843 mL 1.1686 mL 2.9214 mL
40 mM 0.0876 mL 0.4382 mL 0.8764 mL 2.1911 mL
50 mM 0.0701 mL 0.3506 mL 0.7011 mL 1.7528 mL
60 mM 0.0584 mL 0.2921 mL 0.5843 mL 1.4607 mL
80 mM 0.0438 mL 0.2191 mL 0.4382 mL 1.0955 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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p-Nitrophenyl α-L-fucoside Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

p-Nitrophenyl α-L-fucoside10231-84-2PNP α-L-FucBiochemical Assay Reagentsα-L-fucosidasesubstrateInhibitorinhibitorinhibit

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