2. Biochemical Assay Reagents
  3. Enzyme Substrates

Enzyme Substrates

Enzyme substrates are compounds that enzymes act upon and catalyze, The specific binding between the enzyme and the substrate forms an enzyme-substrate complex, resulting in color or fluorescence changes that facilitate detection.

MCE provides highly sensitive and specific enzyme substrates, including chromogenic and fluorescent substrates.

Enzyme Substrates (233):

Cat. No. Product Name CAS No. Purity Chemical Structure
  • HY-126388
    Trypsin Inhibitor, soybean 9035-81-8
    Trypsin Inhibitor, soybean is a potent and reversible inhibitor of trypsin.
    Trypsin Inhibitor, soybean
  • HY-116285
    n-Octyl β-D-glucopyranoside 29836-26-8 99.92%
    n-Octyl-β-d-glucopyranoside is a non-ionic detergent, it can be widely used in the research of biotechnical, biochemical applications, solubilization and crystallization of membrane proteins. n-Octyl-β-d-glucopyranoside can completely inhibit cavitation-induced cell lysis in vitro.
    n-Octyl β-D-glucopyranoside
  • HY-D0714
    Tetrazolium Red 298-96-4 99.93%
    Tetrazolium Red (2,3,5-Triphenyltetrazolium chloride; TTC) is a not brain-penetrant, colorless, water-soluble dye that is reduced by mitochondrial enzymes to a deep red, water-insoluble compound (formazan) mainly in the mitochondria of living cells. Tetrazolium Red is used to observe the activity of dehydrogenase, and it turns colorless to red when exposed to hydrogen. Tetrazolium Red distinguishes between surviving and infarcted brain tissue after stroke. Tetrazolium Red has been used to stain heart tissue to measure the extent of acute lesions and also used to stain brain tissue to detect the size of the infarcted area. The absorption wavelength of Tetrazolium Red is 570 nm.
    Tetrazolium Red
  • HY-15926
    ONPG 369-07-3 99.91%
    ONPG is a colorimetric and spectrophotometric substrate for detection of β-galactosidase activity.
    ONPG
  • HY-B0445A
    NAD sodium 20111-18-6 99.07%
    NAD (β-Nicotinamide Adenine Dinucleotide) sodium is an analogue of NAD. NAD sodium can be reduced to β-nicotinamide adenine dinucleotide (NADH) during coupling with reactions which oxidize organic substrates. NAD sodium can be converted to β-nicotinamide adenine dinucleotide (NADH) and passes to the inside of mitochondria that indirectly generates ATP.
    NAD sodium
  • HY-W286423
    L-Pyroglutamic acid 7-amido-4-methylcoumarin 66642-36-2
    L-Pyroglutamic acid 7-amido-4-methylcoumarin is a fluorogenic substrate for pyroglutamate aminopeptidase 1 (PGP-1).
    L-Pyroglutamic acid 7-amido-4-methylcoumarin
  • HY-W291944
    5-Bromo-4-chloro-3-indolyl-α-L-fucopyranoside 171869-92-4
    5-Bromo-4-chloro-3-indolyl-α-L-fucopyranoside can be used as a chromogenic substrate for α-L-Fucosidase.
    5-Bromo-4-chloro-3-indolyl-α-L-fucopyranoside
  • HY-137198
    4-Nitrophenyl β-D-maltoside 56846-39-0
    4-Nitrophenyl β-D-maltoside is a β-maltosidase substrate that can be used in glycobiology research.
    4-Nitrophenyl β-D-maltoside
  • HY-D0719
    Fluorescein Diacetate 596-09-8 99.79%
    Fluorescein diacetate is a cell permeable esterase-substrate. Fluorescein diacetate can be used as a fluorogenic substrate for hGSTP1-1.
    Fluorescein Diacetate
  • HY-15927
    4-Nitrophenyl β-D-glucopyranoside 2492-87-7 99.69%
    4-Nitrophenyl β-D-glucopyranoside is a chromogenic substrate for β-glucosidase. 4-Nitrophenyl β-D-glucopyranoside is converted to a colored product, p-nitrophenol that is easily detected spectrophotometrically at 405 nm when used in a β-glycosidase assay. 4-Nitrophenyl β-D-glucopyranoside is hydrolysed through intramolecular nucleophilic catalysis by the phosphate group in the 2-position. 4-Nitrophenyl β-D-glucopyranoside is promising for research of postmenopausal osteoporosis.
    4-Nitrophenyl β-D-glucopyranoside
  • HY-W014449
    4-Nitrophenyl butyrate 2635-84-9 99.93%
    4-Nitrophenyl butyrate consists of butyric acid chains esterified with 4-nitrophenol groups, thus giving it a yellow color. This compound is commonly used as a substrate in enzyme assays to measure esterase and lipase activity. When these enzymes cleave the ester bond, the nitrophenol group is released and the color changes from yellow to orange. Thus, the rate of color change can be used to determine enzyme activity. In addition, 4-Nitrophenyl butyrate can also be used as organic synthesis reagent and dye intermediate.
    4-Nitrophenyl butyrate
  • HY-D0183
    ATP-polyamine-biotin 1800401-93-7 98.06%
    ATP-polyamine-biotin, the first cell-permeable ATP analogue, is an efficient kinase cosubstrate. ATP-polyamine-biotin promotes biotin labeling of kinase substrates in live cells.
    ATP-polyamine-biotin
  • HY-W011012
    Adenosine 5'-monophosphate disodium 4578-31-8 99.63%
    Adenosine 5'-monophosphate disodium is an orally active purine nucleotide, and participates in ATP metabolism. Adenosine 5'-monophosphate disodium is also a ligand for adenosine 2B receptor. Adenosine 5'-monophosphate disodium can activate AMPK in skeletal muscle, and ameliorates insulin resistance and impaired glucose metabolism. Adenosine 5'-monophosphate disodium can be used for research of diabetes.
    Adenosine 5'-monophosphate disodium
  • HY-134426
    DL-β-Hydroxybutyryl coenzyme A lithium 103404-51-9 98.00%
    DL-β-Hydroxybutyryl coenzyme A lithium is an intermediate in the fermentation of butyric acid and the metabolism of lysine and tryptophan, and is produced from β-hydroxybutyric acid by short-chain-CoA synthase.
    DL-β-Hydroxybutyryl coenzyme A lithium
  • HY-116022A
    4-Nitrophenyl phosphate disodium hexahydrate 333338-18-4 99.80%
    4-Nitrophenyl phosphate (p-nitrophenyl phosphate) disodium hexahydrate is widely used as a small molecule phosphotyrosine-like substrate in activity assays for protein tyrosine phosphatases. 4-Nitrophenyl phosphate disodium hexahydrate is a colorless substrate that upon hydrolysis is converted to a yellow 4-nitrophenolate ion that can be monitored by absorbance at 405 nm.
    4-Nitrophenyl phosphate disodium hexahydrate
  • HY-128851B
    Coenzyme A sodium 55672-92-9 99.46%
    Coenzyme A (CoASH) sodium is a ubiquitous and essential cofactor, which is an acyl group carrier and carbonyl-activating group for the citric acid cycle and fatty acid metabolism. Coenzyme A plays a central role in the oxidation of pyruvate in the citric acid cycle and the metabolism of carboxylic acids, including short- and long-chain fatty acids.
    Coenzyme A sodium
  • HY-D0904
    Acetylthiocholine iodide 1866-15-5 99.87%
    Acetylthiocholine iodide can be used as a substrate for certain enzymes, such as cholinesterase, etc., and can be used to determine the activity level of these enzymes. In addition, the compound is used in some medical research, for example in the fields of neuroscience and organ physiology.
    Acetylthiocholine iodide
  • HY-W017386
    3-Methyl-2-oxovaleric acid sodium 3715-31-9 99.60%
    3-Methyl-2-oxovaleric acid sodium is a degradation product from Isoleucine. 3-Methyl-2-oxovaleric acid sodium is a biomarker of mustard airway diseases (MADs) and uric acid stone.
    3-Methyl-2-oxovaleric acid sodium
  • HY-137855
    4-Methylumbelliferyl sulfate potassium 15220-11-8 99.29%
    4-Methylumbelliferyl sulfate (potassium), a fluorescent substrate, is commonly used to detect sulfatase activity in biochemical and biomedical research. It consists of a sulfate group attached to a fluorescent molecule, which can be cleaved by sulfatase enzymes. Upon cleavage, 4-Methylumbelliferyl sulfate releases a highly fluorescent product that can be detected using fluorescence microscopy or spectroscopy. The use of 4-Methylumbelliferyl sulfate as a substrate for sulfatase enzymes allows accurate detection and quantification of these enzymes in a variety of biological samples.
    4-Methylumbelliferyl sulfate potassium
  • HY-W016412
    Coenzyme Q0 605-94-7 99.88%
    Coenzyme Q0 (CoQ0) is a potent, oral active ubiquinone compound can be derived from Antrodia cinnamomea. Coenzyme Q0 induces apoptosis and autophagy, suppresses of HER-2/AKT/mTOR signaling to potentiate the apoptosis and autophagy mechanisms. Coenzyme Q0 regulates NFκB/AP-1 activation and enhances Nrf2 stabilization in attenuation of inflammation and redox imbalance. Coenzyme Q0 has anti-angiogenic activity through downregulation of MMP-9/NF-κB and upregulation of HO-1 signaling.
    Coenzyme Q0