2. Others Apoptosis PI3K/Akt/mTOR
  3. Biochemical Assay Reagents Survivin Bcl-2 Family Caspase Apoptosis mTOR Akt
  4. N-Hydroxyphthalimide

N-Hydroxyphthalimide is a blocking agent and catalyst. N-Hydroxyphthalimide promotes oxidation reactions by generating PINO free radicals and activating hydrogen atom transfer processes. N-Hydroxyphthalimide reduces the expression of anti-apoptotic proteins Survivin and Bcl-xL and activates caspase 9 and caspase 3. N-Hydroxyphthalimide induces Apoptosis. N-Hydroxyphthalimide inhibits the phosphorylation of mTOR (Ser2448, Ser2481) and Akt (Ser473). N-Hydroxyphthalimide has anticancer effects against breast and colon cancer.

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N-Hydroxyphthalimide

N-Hydroxyphthalimide Chemical Structure

CAS No. : 524-38-9

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Other Forms of N-Hydroxyphthalimide:

N-Hydroxyphthalimide Related Antibodies

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  • Biological Activity

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  • References

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Description

N-Hydroxyphthalimide is a blocking agent and catalyst. N-Hydroxyphthalimide promotes oxidation reactions by generating PINO free radicals and activating hydrogen atom transfer processes. N-Hydroxyphthalimide reduces the expression of anti-apoptotic proteins Survivin and Bcl-xL and activates caspase 9 and caspase 3. N-Hydroxyphthalimide induces Apoptosis. N-Hydroxyphthalimide inhibits the phosphorylation of mTOR (Ser2448, Ser2481) and Akt (Ser473). N-Hydroxyphthalimide has anticancer effects against breast and colon cancer[1][2][3].

IC50 & Target[3]

Caspase 9

 

Caspase 3

 

Bcl-xL

 

In Vitro

N-Hydroxyphthalimide (2.5-40 μM; 24-72 h) selectively inhibits the proliferation of human breast carcinoma BT-20 cells, human colon adenocarcinoma LoVo and HT-29 cells by inducing G2/M phase arrest and apoptosis[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

N-Hydroxyphthalimide Related Antibodies

Western Blot Analysis[3]

Cell Line: Decreased the expressions of anti-apoptotic proteins survivin and Bcl-xL.
Activated caspase 9 and caspase 3.
BT-20, LoVo cells
Concentration: 2.5 μM, 5 μM, 10 μM, 20 μM
Incubation Time: 24 h
Result: Decreased the expressions of anti-apoptotic proteins survivin and Bcl-xL.
Activated caspase 9 and caspase 3.
Suppressed the phosphorylation of mTOR (Ser2448, Ser2481).
In Vivo

N-Hydroxyphthalimide (40 mg/kg; i.p.; 53 days) significantly inhibits tumor growth in BT-20 xenograft models by suppressing both mTORC1 and mTORC2 signaling pathways, without causing obvious toxicity to nude mice[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Three-week old female BALB/c nude mice; human breast carcinoma BT-20 cell xenograft model[3]
Dosage: 40 mg/kg
Administration: Intraperitoneal injection; 53 days (started when tumor volume reached approximately 100 mm3)
Result: Significantly reduced tumor volume and tumor weight.
Achieved a 53% tumor growth inhibition rate.
Decreased the tumor weight by 45%.
Decreased the phosphorylation levels of mTOR at Ser2448, S6 at Ser235/236, 4E-BP1 at Ser65, and Akt at Ser473.
Molecular Weight

163.13

Formula

C8H5NO3
CAS No.
Appearance

Solid

Color

White to light yellow

SMILES

O=C1C2=C(C(N1O)=O)C=CC=C2
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (613.01 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 6.1301 mL 30.6504 mL 61.3008 mL
5 mM 1.2260 mL 6.1301 mL 12.2602 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

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In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation

Purity: 99.89%

SDS (393 KB)

COA (254 KB) HNMR (113 KB) RP-HPLC (191 KB) LCMS (257 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 6.1301 mL 30.6504 mL 61.3008 mL 153.2520 mL
5 mM 1.2260 mL 6.1301 mL 12.2602 mL 30.6504 mL
10 mM 0.6130 mL 3.0650 mL 6.1301 mL 15.3252 mL
15 mM 0.4087 mL 2.0434 mL 4.0867 mL 10.2168 mL
20 mM 0.3065 mL 1.5325 mL 3.0650 mL 7.6626 mL
25 mM 0.2452 mL 1.2260 mL 2.4520 mL 6.1301 mL
30 mM 0.2043 mL 1.0217 mL 2.0434 mL 5.1084 mL
40 mM 0.1533 mL 0.7663 mL 1.5325 mL 3.8313 mL
50 mM 0.1226 mL 0.6130 mL 1.2260 mL 3.0650 mL
60 mM 0.1022 mL 0.5108 mL 1.0217 mL 2.5542 mL
80 mM 0.0766 mL 0.3831 mL 0.7663 mL 1.9157 mL
100 mM 0.0613 mL 0.3065 mL 0.6130 mL 1.5325 mL
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N-Hydroxyphthalimide Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

N-Hydroxyphthalimide524-38-9Biochemical Assay ReagentsSurvivinBcl-2 FamilyCaspaseApoptosismTORAktMammalian target of RapamycinPKBProtein kinase BBALB/cnude miceBT-20LoVoHT-29anticancerInhibitorinhibitorinhibit

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