MMRi6 

MMRi6 is a Mdm2-MdmX RING domain inhibitor that can disrupt Mdm2-MdmX RING-RING interaction in vitro. MMRi6 inhibits MdmX-stimulated Mdm2 autoubiquitination and Mdm2-MdmX-mediated p53 polyubiquitination in vitro without affecting NEDD4-1 autoubiquitination. MMRi6 induces p53 stabilization and accumulation and induces PARP cleavage in wt-p53 Emu-myc lymphoma cells. MMRi6 inhibits the growth of wt-p53 and p53-null Emu-myc lymphoma cells with IC₅₀s of approximately 0.5 μM and 3 μM, respectively. MMRi6 can be used for the study of leukemia/lymphoma^[1].

MMRi6 (10 μM) inhibits Mdm2 autoubiquitination and p53 ubiquitination by Mdm2–MdmX in vitro^[1].
MMRi6 (5 μM, 8 h) induces p53 protein accumulation in HCT-8 cells and in wt-p53 Emu-myc lymphoma cells^[1].
MMRi6 (0.5 μM, 24 h) induces PARP cleavage in wt-p53 Emu-myc lymphoma cells but not in p53-null Emu-myc lymphoma cells^[1].
MMRi6 (0.5-1 μM, 72 h) inhibits the growth of wt-p53 and p53-null Emu-myc lymphoma cells with IC₅₀s of approximately 0.5 μM and 3 μM, respectively^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

396.27

C₂₁H₁₅Cl₂N₃O

709009-15-4

Solid

OC1=C2N=CC=CC2=C(Cl)C=C1C(C3=CC=CC(Cl)=C3)NC4=NC=CC=C4

Room temperature in continental US; may vary elsewhere.

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

• [1]. Wu W, et al. Targeting RING domains of Mdm2-MdmX E3 complex activates apoptotic arm of the p53 pathway in leukemia/lymphoma cells. Cell Death Dis. 2015 Dec 31;6(12):e2035.  [Content Brief]