2. Immunology/Inflammation
  3. CD74
  4. Milatuzumab

Milatuzumab  (Synonyms: hLL1; MEDI-115)

Cat. No.: HY-P99731 Purity: 99.39%
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Milatuzumab (hLL1; MEDI-115) is a humanized anti-CD74 monoclonal antibody. CD74, a integral membrane protein, is associated with the promotion of B-cell growth and survival. Milatuzumab causes free radical oxygen generation, and loss of mitochondrial membrane potential. Milatuzumaba also decreases CD20/CD74 aggregates and cell adhesion, to lead to cell death.

For research use only. We do not sell to patients.

CAS No. : 899796-83-9

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Description

Milatuzumab (hLL1; MEDI-115) is a humanized anti-CD74 monoclonal antibody. CD74, a integral membrane protein, is associated with the promotion of B-cell growth and survival. Milatuzumab causes free radical oxygen generation, and loss of mitochondrial membrane potential. Milatuzumaba also decreases CD20/CD74 aggregates and cell adhesion, to lead to cell death[1].

Isotype

Human IgG1 kappa
Recommend Isotype Controls
IC50 & Target

CD74
In Vitro

Milatuzumaba (5 μg/mL; 8-48 h) enhances cell death in MCL cell lines and primary patient tumor cells[1].
Milatuzumaba (5 μg/mL; 0.5-2 h) mediates the cytotoxicity partially depending on generation of ROS and loss of mitochondrial transmembrane potential in Jeko, Mino, and SP-53 cells[1].
Milatuzumaba (5 μg/mL; 4 h) inhibits NF-κB pathway and induces cell apoptosis with independent of caspase cleavage, Bcl-2 family member dysregulation, or induction of autophagy[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Milatuzumab Related Antibodies

Western Blot Analysis[1]

Cell Line: Jeko and Mino cells
Concentration: 5 μg/mL
Incubation Time: 4 hours
Result: Insignificant down-regulation of antiapoptotic proteins, such as Bax, Bcl-2, Bcl-xL, and Mcl-1.

Cell Viability Assay[1]

Cell Line: MCL cell lines and primarypatient tumor cells
Concentration: 5 μg/mL
Incubation Time: 8, 24, and 48 hours
Result: Resulted in cell death of Jeko, Mino, SP-53, Rec-1, HBL-2, and Granta cells.

Immunofluorescence[1]

Cell Line: Jeko, Mino, and SP-53 cells
Concentration: 5 μg/mL; with or without 10 mM N-acetylcysteine (HY-B0215) for 1.5 h
Incubation Time: 0.5, 1, 1.5, and 2 hours
Result: Increased ROS generation as early as 0.5 hours, while peaking at 1 to 1.5 hours and reducing at 2 hours.Therefore, it resulted cell death, but reserved by nonspecific ROS scavenger.
In Vivo

Milatuzumaba (15 mg/kg/day; i.p.; once every 3 days) significantly increases the survival rate of female SCID mice bearing Jeko cells. And Milatuzumaba has a synergistic effect with Rituximab (HY-P9913) in mouse model[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Jeko mouse model[1]
Dosage: 15 mg/kg/day; with or without 15 mg/kg Rituximab
Administration: Intraperitoneal injection; once every 3 days, starting at day 15 after engraftment
Result: Resulted the mean survival for the combination treated group of 44.5 days, compared with 33.5 days for Milatuzumaba treated, 28 days for control.
Clinical Trial
Gene ID

972  [NCBI]

Accession
Application

ELISA, FACS, Functional assay
Conjugated

Unconjugated
Reconsititution

The product can be reconstituted/diluted with sterile PBS or saline.
Molecular Weight

145.66 kDa

CAS No.
Appearance

Liquid

Color

Colorless to light yellow

SMILES

[Milatuzumab]
Shipping

Shipping with dry ice.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.
Format
  • Human IgG1 kappa
Biological Activity
  • Immobilized hu-CD74-ECD-His can bind Milatuzumab. The EC50 for this effect is 3.941 ng/mL.
Purity & Documentation

Purity: 99.39%

SDS (251 KB)

COA (232 KB)

Inhibitory Antibodies User Guide (602 KB)
References
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Milatuzumab Related Classifications

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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Milatuzumab899796-83-9hLL1 MEDI-115hLL 1hLL-1MEDI115MEDI 115MEDI-115CD74HLA-DR-gammaInvariant polypeptide of major histocompatibility complex, class II antigen-associatedInhibitorinhibitorinhibit

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