2. Anti-infection Metabolic Enzyme/Protease Apoptosis
  3. Bacterial MMP Apoptosis Fungal
  4. Methylisothiazolinone hydrochloride

Methylisothiazolinone hydrochloride 

Cat. No.: HY-W010243 Purity: 99.40%
COA
SDS
Handling Instructions Technical Support

Methylisothiazolinone hydrochloride is a bacterial and fungal inhibitor and preservative, as well as a sensitizer. Methylisothiazolinone hydrochloride can activate matrix metalloproteinases (MMPs) in human bronchial epithelial cells to induce apoptosis and inflammatory response. Methylisothiazolinone hydrochloride can promote the development of atopic dermatitis in mice by disrupting Th2/Th17 related immune responses. Methylisothiazolinone hydrochloride can cause mitochondrial damage in the endothelium of rat cerebral blood vessels.

For research use only. We do not sell to patients.

Methylisothiazolinone hydrochloride

Methylisothiazolinone hydrochloride Chemical Structure

CAS No. : 26172-54-3

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Based on 1 publication(s) in Google Scholar

Other Forms of Methylisothiazolinone hydrochloride:

Methylisothiazolinone hydrochloride Related Antibodies

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  • References

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Description

Methylisothiazolinone hydrochloride is a bacterial and fungal inhibitor and preservative, as well as a sensitizer. Methylisothiazolinone hydrochloride can activate matrix metalloproteinases (MMPs) in human bronchial epithelial cells to induce apoptosis and inflammatory response. Methylisothiazolinone hydrochloride can promote the development of atopic dermatitis in mice by disrupting Th2/Th17 related immune responses. Methylisothiazolinone hydrochloride can cause mitochondrial damage in the endothelium of rat cerebral blood vessels[1][2][3][4].
In Vitro

Methylisothiazolinone (0-8 μg/mL, 24 h) hydrochloride induces cell apoptosis and inflammatory response by activating matrix metalloproteinases (MMPs) in human bronchial epithelial cells[1].
Methylisothiazolinone (0-2.5 μg/mL, 1 h) hydrochloride can cause mitochondrial damage in brain endothelial cells[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Methylisothiazolinone hydrochloride Related Antibodies

Cell Viability Assay[1]

Cell Line: Bronchial epithelial cells (BEAS-2B cells)
Concentration: 0, 2, 4, 8 μg/mL
Incubation Time: 24 h
Result: Caused a decrease in cell viability to 81.0%, 67.9%, and 35.1% at doses of 2, 4, and 8 μg/mL, respectively.

Western Blot Analysis[1]

Cell Line: bEND.3 cell
Concentration: 0, 1, 2.5 μg/mL CMIT/MIT
Incubation Time: 1 h
Result: Increased the level of Drp-1 phosphorylated protein and decreased the level of Mfn-1 phosphorylated protein.
In Vivo

Methylisothiazolinone (0.15 mg/kg; once; i.v.) hydrochloride can increase the susceptibility of rat cerebral vascular endothelium to endogenous pathological stimuli and lead to mitochondrial damage[2].
Methylisothiazolinone (20 or 40 μL; handling skin (20 μL), days 1 and 8; intranasal instillation (40 μL), day 15) hydrochloride can cause sensitization in asthmatic mice when handled on the skin[3].
Methylisothiazolinone (0.1875 mg/kg CMIT/MIT; once daily; 15 days; epidermal contact) hydrochloride promotes the development of atopic dermatitis in mice through Th2/Th17 related immune response dysregulation[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Male SD rats (230-300 g and 7-8 weeks of age)[2]
Dosage: 0.15 mg/kg
Administration: Intravenous injection (i.v.); once
Result: Resulted in mitochondrial damage and a decrease in tight junction proteins.
Animal Model: Male C57Bl/6 and BALB/c mouse asthma models induced by chemistry (approximately 20 g, 6 weeks old)[3]
Dosage: 20 or 40 μL
Administration: Handled skin (20 μL), on days 1 and 8; intranasal instillation (40 μL), on day 15
Result: Induced an increase in T and B cell proliferation in ear lymph nodes, while producing IFN-γ.
Animal Model: Specific dermatitis (AD) female BALB/c mice established by sensitization of ovalbumin (OVA) epidermis (5 weeks old, n = 5 per group) weighing 16-20 g[4]
Dosage: 0.1875 mg/kg chloromethylisothiazolinone/methylisothiazolinone (CMIT/MIT)
Administration: Epidermal contact; once daily; 15 days
Result: Augmented the mRNA expression level of T helper (Th) 2-related cytokines (thymic stromal lymphopoietin, interleukin (IL)-6 and IL-13), Th2 chemokine (chemokine (C-C motif) ligand 17) and the population of CD4IL-4 cells in the skin.
Molecular Weight

151.61

Formula

C4H6ClNOS
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

O=C1N(C)SC=C1.[H]Cl
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (659.59 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 6.5959 mL 32.9794 mL 65.9587 mL
5 mM 1.3192 mL 6.5959 mL 13.1917 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

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In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation

Purity: 99.40%

SDS (786 KB)

COA (255 KB) HNMR (115 KB) RP-HPLC (196 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 6.5959 mL 32.9794 mL 65.9587 mL 164.8968 mL
5 mM 1.3192 mL 6.5959 mL 13.1917 mL 32.9794 mL
10 mM 0.6596 mL 3.2979 mL 6.5959 mL 16.4897 mL
15 mM 0.4397 mL 2.1986 mL 4.3972 mL 10.9931 mL
20 mM 0.3298 mL 1.6490 mL 3.2979 mL 8.2448 mL
25 mM 0.2638 mL 1.3192 mL 2.6383 mL 6.5959 mL
30 mM 0.2199 mL 1.0993 mL 2.1986 mL 5.4966 mL
40 mM 0.1649 mL 0.8245 mL 1.6490 mL 4.1224 mL
50 mM 0.1319 mL 0.6596 mL 1.3192 mL 3.2979 mL
60 mM 0.1099 mL 0.5497 mL 1.0993 mL 2.7483 mL
80 mM 0.0824 mL 0.4122 mL 0.8245 mL 2.0612 mL
100 mM 0.0660 mL 0.3298 mL 0.6596 mL 1.6490 mL
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Methylisothiazolinone hydrochloride Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Methylisothiazolinone26172-54-3BacterialMMPApoptosisFungalMatrix metalloproteinasessterilizationallergymitochondrial damagedermatitisInhibitorinhibitorinhibit

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