Methyl pheophorbide a

Cat. No.: HY-N10902 Purity: 99.57%: FAQs
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Methyl pheophorbide a is a natural product, one of the chlorophyll-a derivatives. Methyl pheophorbide a is a photosensitizer that produces singlet oxygen and reactive oxygen species (ROS) under light of specific wavelength, thereby leading to cell death.

For research use only. We do not sell to patients.

Methyl pheophorbide a Chemical Structure

CAS No. : 5594-30-9

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1 mg	In-stock	Estimated Time of Arrival: December 31
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Biological Activity
Purity & Documentation
References
Customer Review

Description

Cellular Effect

Cell Line	Type	Value	Description	References
1A9	ED₅₀	0.26 μg/mL Compound: 5	Cytotoxicity against human 1A9 cells after 72 hrs by SRB assay Cytotoxicity against human 1A9 cells after 72 hrs by SRB assay	[PMID: 11473423]
A549	ED₅₀	0.83 μg/mL Compound: 5	Cytotoxicity against human A549 cells after 72 hrs by SRB assay Cytotoxicity against human A549 cells after 72 hrs by SRB assay	[PMID: 11473423]
Daudi	IC₅₀	25 μM Compound: 6, mPheo	Cytotoxicity against human Daudi cells under deem light after 96 hrs by MTT assay Cytotoxicity against human Daudi cells under deem light after 96 hrs by MTT assay	[PMID: 17993275]
HCT-8	ED₅₀	1 μg/mL Compound: 5	Cytotoxicity against human HCT8 cells after 72 hrs by SRB assay Cytotoxicity against human HCT8 cells after 72 hrs by SRB assay	[PMID: 11473423]
HeLa	IC₅₀	0.182 μM Compound: 7, MPa	Photosensitizing activity against human HeLa cells assessed as reduction in cell viability pre-incubated for 24 hrs followed by 15 mins exposure to 670 to 710 nm light at dose of 2.0 J/cm2 and measured 12 hrs after radiation by WST-8 assay Photosensitizing activity against human HeLa cells assessed as reduction in cell viability pre-incubated for 24 hrs followed by 15 mins exposure to 670 to 710 nm light at dose of 2.0 J/cm2 and measured 12 hrs after radiation by WST-8 assay	[PMID: 25797162]
HeLa	IC₅₀	25 μM Compound: 6, mPheo	Cytotoxicity against human HeLa cells under deem light after 96 hrs by MTT assay Cytotoxicity against human HeLa cells under deem light after 96 hrs by MTT assay	[PMID: 17993275]
K562	IC₅₀	> 100 μM Compound: 6, mPheo	Cytotoxicity against human K562 cells under deem light after 96 hrs by MTT assay Cytotoxicity against human K562 cells under deem light after 96 hrs by MTT assay	[PMID: 17993275]
KB	ED₅₀	0.23 μg/mL Compound: 5	Cytotoxicity against human KB cells after 72 hrs by SRB assay Cytotoxicity against human KB cells after 72 hrs by SRB assay	[PMID: 11473423]
MCF7	ED₅₀	0.95 μg/mL Compound: 5	Cytotoxicity against human MCF7 cells after 72 hrs by SRB assay Cytotoxicity against human MCF7 cells after 72 hrs by SRB assay	[PMID: 11473423]
NCI-H69	IC₅₀	> 100 μM Compound: 6, mPheo	Cytotoxicity against human H69 cells under deem light after 96 hrs by MTT assay Cytotoxicity against human H69 cells under deem light after 96 hrs by MTT assay	[PMID: 17993275]
Raji	IC₅₀	> 100 μM Compound: 6, mPheo	Cytotoxicity against human Raji cells under deem light after 96 hrs by MTT assay Cytotoxicity against human Raji cells under deem light after 96 hrs by MTT assay	[PMID: 17993275]
SiHa	IC₅₀	> 100 μM Compound: 6, mPheo	Cytotoxicity against human SIHA cells under deem light after 96 hrs by MTT assay Cytotoxicity against human SIHA cells under deem light after 96 hrs by MTT assay	[PMID: 17993275]
SK-MEL	ED₅₀	1.2 μg/mL Compound: 5	Cytotoxicity against human SK-MEL cells after 72 hrs by SRB assay Cytotoxicity against human SK-MEL cells after 72 hrs by SRB assay	[PMID: 11473423]

In Vitro

Methyl pheophorbide a (0.25-2 μg/mL, 4 h) reduces the cell viability of U937 and SK-HEP-1, activates caspase 3/7, causes the formation of apoptotic bodies and vesicle, and induces apoptosis^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[3]

Cell Line:	U937 and SK-HEP-1
Concentration:	0.25-2 μg/mL
Incubation Time:	4 h
Result:	Inhibited cell viability under light.

Molecular Weight

606.71

Formula

C₃₆H₃₈N₄O₅

CAS No.

5594-30-9

Appearance

Solid

Color

Brown to black

SMILES

COC([C@@H]1/C2=C([C@H]([C@@H]3C)CCC(OC)=O)/N=C3/C=C(C(C)=C/4C=C)\NC4=C/C5=N/C(C(CC)=C5C)=C\C6=C(C)C(C1=O)=C2N6)=O

Structure Classification

Initial Source

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
In solvent	-80°C	6 months
	-20°C	1 month

Purity & Documentation

Purity: 99.57%

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Data Sheet (279 KB) SDS (251 KB)

COA (261 KB) HNMR (272 KB) RP-HPLC (303 KB) MS (69 KB)

Handling Instructions (2659 KB)

References

[1]. F.C. Pennington, et al. Preparation and Properties of Pyrochlorophyll a, Methyl Pyrochlorophyllide a, Pyropheophytin a, and Methyl Pyropheophorbide a Derived from Chlorophyll by Decarbomethoxylation. J. Am. Chem. Soc. 1964, 86, 7, 1418-1426.

[2]. ShinOgasawara, et al. C132-Methylation of methyl pheophorbide a and stereoselective preparation of methyl (132R)-methylpyropheophorbide a. Tetrahedron Letters, 2014, 3618-3621.

[3]. Ha J Y, et al. Isolation, identification, and apoptosis activity of the photosensitizer methyl pheophorbide A from Perilla frutescens leaves[J]. Applied Biological Chemistry, 2022, 65(1): 52.