2. Academic Validation
  3. Oroxin A suppresses colorectal tumor growth by regulating the TRIM24-mediated ferroptosis and TSPO pathway

Oroxin A suppresses colorectal tumor growth by regulating the TRIM24-mediated ferroptosis and TSPO pathway

  • iScience. 2025 Jul 24;28(8):113196. doi: 10.1016/j.isci.2025.113196.
Chenghai He 1,  Chunyan Weng 2,  Zhichao Lai 1,  Hui Luo 1,  Kexin Chen 1,  Tangliang Li 1,  Weimin Li 1,  Guodong Li 1
Affiliations

Affiliations

  • 1 Department of Gastroenterology, The Affiliated Hospital of Hangzhou Normal University, Hangzhou 310015, Zhejiang Province, China.
  • 2 The First Clinical Medical of Zhejiang Chinese Medicine University, Hangzhou, Zhejiang, China.
PMID: 40822356 DOI: 10.1016/j.isci.2025.113196
Abstract

Colorectal Cancer (CRC) is a common malignancy, and TRIM24, an E3 ubiquitin Ligase, is a potential target for various cancers. This study found high TRIM24 expression in CRC, correlating with poor prognosis and disease progression. Oroxin A (OA) was identified as a TRIM24 inhibitor, promoting its degradation and inhibiting CRC cell proliferation, invasion, and migration in vitro. OA treatment linked to ferroptosis-related protein SLC3A2 and angiogenesis regulator TSPO, modulating the expression of key Ferroptosis markers in a TRIM24-dependent manner. OA downregulated TRIM24, affecting TSPO ubiquitination and suppressing angiogenesis. In vivo, OA inhibited CRC tumor growth with minimal toxicity. OA is a potent antitumor agent targeting TRIM24, inducing Ferroptosis, and inhibiting angiogenesis.

Keywords

Cancer; Cell biology; Pharmacology.

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