2. Academic Validation
  3. Transcriptional Analysis of Spodoptera frugiperda Sf9 Cells Infected with Daphnis nerii Cypovirus-23

Transcriptional Analysis of Spodoptera frugiperda Sf9 Cells Infected with Daphnis nerii Cypovirus-23

  • Int J Mol Sci. 2025 Aug 2;26(15):7487. doi: 10.3390/ijms26157487.
Wendong Kuang 1 2 3,  Jian Yang 1 2,  Jinchang Wang 1 2,  Chenghua Yan 4,  Junhui Chen 1 2,  Xinsheng Liu 5,  Chunhua Yang 2,  Zhigao Zhan 1 2,  Limei Guan 1 2,  Jianghuai Li 1 2,  Tao Deng 1,  Feiying Yang 2,  Guangqiang Ma 4,  Liang Jin 1 2
Affiliations

Affiliations

  • 1 Institute of Microbiology, Jiangxi Academy of Sciences, Nanchang 330029, China.
  • 2 Institute of Biological Resources, Jiangxi Academy of Sciences, Nanchang 330029, China.
  • 3 School of Life Sciences, Jiangxi University of Traditional Chinese Medicine, Nanchang 330004, China.
  • 4 State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences (CAS), Wuhan 430071, China.
  • 5 State Key Laboratory for Animal Disease Control and Prevention, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730050, China.
PMID: 40806613 DOI: 10.3390/ijms26157487
Abstract

Daphnis nerii cypovirus-23 (DnCPV-23) is a new type of cypovirus that has a lethal effect on many species of Sphingidae pests. DnCPV-23 can replicate in Spodoptera frugiperda Sf9 cells, but the replication characteristics of the virus in this cell line are still unclear. To determine the replication characteristics of DnCPV-23 in Sf9 cells, uninfected Sf9 cells and Sf9 cells at 24 and 72 h after DnCPV-23 Infection were collected for transcriptome analysis. Compared to uninfected Sf9 cells, a total of 188 and 595 differentially expressed genes (DEGs) were identified in Sf9 cells collected at 24 hpi and 72 h, respectively. KEGG analyses revealed that 139 common DEGs in two treatment groups were related to nutrition and energy metabolism-related processes, cell membrane integrity and function-related pathways, detoxification-related pathways, growth and development-related pathways, and so on. We speculated that these cellular processes might be manipulated by viruses to promote replication. This study provides an important basis for further in-depth research on the mechanism of interaction between viruses and hosts. It provides additional basic information for the future exploitation of DnCPV-23 as a biological Insecticide.

Keywords

Daphnis nerii cypovirus-23; Sf9 cells; transcriptome analysis.

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