Novel β-carboline derivatives show promise as dual-target inhibitors of DNA and TOP2A for the treatment of triple negative breast cancer

Breast Cancer is a leading cause of death among women globally, with triple-negative breast Cancer (TNBC) being the most aggressive subtype. To develop effective and safe chemotherapeutic drugs, we identified TOP2A as a potential target due to its differential expression between breast Cancer and normal tissues. By targeting hydrogen bond-van der Waals sites, we discovered three active amino acid sites in TOP2A (E461, D463, and D543) and designed a β-carboline derivative, DM1, targeting DNA and TOP2A using a molecular generation model, local docking, and optimization. Molecular dynamics simulations showed that DM1 binds effectively to DNA and TOP2A, with its amide linker co-anchoring E461 and D543 alongside Mg²⁺, while the β-carboline ring interacts with DNA and stabilizes the connection with D463. This binding disrupts Mg²⁺-mediated ATP stabilization and blocks DNA reconnection. DM1 shows strong antiproliferative activity against 4T1 cells (IC₅₀ = 1.12 ± 0.13 μM), outperforming etoposide (IC₅₀ = 9.37 ± 0.52 μM), and inhibits cell migration. Further experiments demonstrated that DM1 selectively binds to TOP2A, not TOP2B, induces DNA damage, and increases Reactive Oxygen Species (ROS) in TNBC cells. Proteomics analysis confirmed that DM1's mechanism involves nucleosomes, replication forks, and heterochromatin related to the two targets. In a 4T1 xenograft model, DM1 achieves a 71.50 % tumor growth inhibition (TGI) at 20 mg/kg, compared to etoposide's 30.93 % TGI at the same dose. DM1 interacts with DNA and TOP2A to form a stable DM1-TOP2A-DNA ternary complex. This blocks DNA's normal function, induces double-strand breaks, causes ROS accumulation, and triggers Apoptosis, thereby inhibiting TNBC progression.

Apoptosis; DNA damage; Molecular docking; ROS; TNBC; TOP2A.