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  3. Quantitative Proteomics Unveils the Synergistic Effects of Combination Drugs on Cytoskeleton Composition and Autophagy-Mediated Cell Death in Neuroblastoma

Quantitative Proteomics Unveils the Synergistic Effects of Combination Drugs on Cytoskeleton Composition and Autophagy-Mediated Cell Death in Neuroblastoma

  • J Proteome Res. 2025 Jul 4;24(7):3578-3596. doi: 10.1021/acs.jproteome.5c00191.
Pei-Chen Yu 1,  Yi-Chun Kao 2,  Hsin-Yi Chang 3,  Chen-Hao Huang 4,  Wen-Ming Hsu 5,  Hsuan-Cheng Huang 6,  Hsueh-Fen Juan 1 2 4 7
Affiliations

Affiliations

  • 1 Institute of Molecular and Cellular Biology, National Taiwan University, Taipei 106, Taiwan.
  • 2 Department of Life Science, National Taiwan University, Taipei 106, Taiwan.
  • 3 Graduate Institute of Medical Sciences, National Defense Medical Center, Taipei 114, Taiwan.
  • 4 Graduate Institute of Biomedical Electronics and Bioinformatics, National Taiwan University, Taipei 106, Taiwan.
  • 5 Department of Surgery, National Taiwan University Hospital and National Taiwan University College of Medicine, Taipei 100, Taiwan.
  • 6 Institute of Biomedical Informatics, National Yang Ming Chiao Tung University, Taipei 112, Taiwan.
  • 7 Center for Computational and Systems Biology, National Taiwan University, Taipei 106, Taiwan.
PMID: 40526398 DOI: 10.1021/acs.jproteome.5c00191
Abstract

Neuroblastoma, a prevalent and aggressive childhood Cancer, lacks effective treatments. Recent research highlights the repurposing of existing drugs as a strategy for breakthroughs in combating this disease. We systematically analyzed small-molecule perturbation gene expression data from the Library of Integrated Network-Based Cellular Signatures (LINCS), identifying pyrvinium pamoate and sirolimus, two FDA-approved drugs, as potential candidates for neuroblastoma combination therapy. Colony formation assays and Organoid culture confirmed that the therapeutic effect of combining these two drugs exceeded that of either drug alone. The mRNA expression levels of several genes predicted by LINCS also decreased. To comprehensively understand the mechanism behind superior efficacy of the combination therapy compared to monotherapy, we performed quantitative proteomics with tandem mass tag labeling and identified 3416 proteins from 20,623 peptides. Gene set enrichment analysis and Database for Annotation, Visualization, and Integrated Discovery revealed that combination therapy significantly decreased Cytoskeleton formation compared with monotherapy, reflecting dramatic reduction in cell migration. Additionally, the research indicated that cell cycle arrest occurred under combination therapy. Furthermore, we confirmed that the extent of Autophagy significantly increased after the combination treatment. In summary, this study elucidates the mechanisms and therapeutic potential of combining sirolimus and pyrvinium pamoate for treating neuroblastoma, offering new advancements for this challenging disease.

Keywords

combination therapy; neuroblastoma; pyrvinium pamoate; sirolimus; tandem mass tag.

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