2. Academic Validation
  3. TRIM65 regulates glucose metabolic reprogramming to promote glioma cell proliferation via ubiquitination and degradation of AMPK

TRIM65 regulates glucose metabolic reprogramming to promote glioma cell proliferation via ubiquitination and degradation of AMPK

  • NPJ Precis Oncol. 2025 Jun 5;9(1):163. doi: 10.1038/s41698-025-00964-z.
Ming-Hui Li # 1 2,  Tao Wang # 1,  Xiao-Hui Guan 3,  Zhen-Ping Yu 3,  Xin-Hao Han 1,  Xin-Hui Qu 1 4,  Zhi-Ping Chen 1,  Xiao-Jian Han 5,  Xiao-Yu Wang 6
Affiliations

Affiliations

  • 1 Institute of Geriatrics, Jiangxi Provincial People's Hospital, The First Affiliated Hospital of Nanchang Medical College, Nanchang, 330006, Jiangxi, PR China.
  • 2 Department of Pharmacology, School of Pharmaceutical Science, Nanchang University, Nanchang, 330006, Jiangxi, PR China.
  • 3 The National Engineering Research Center for Bioengineering Drugs and the Technologies, Institute of Translational Medicine, Nanchang University, Nanchang, 330031, Jiangxi, PR China.
  • 4 The Second Department of Neurology, Jiangxi Provincial People's Hospital, The First Affiliated Hospital of Nanchang Medical College, Nanchang, 330006, Jiangxi, PR China.
  • 5 Institute of Geriatrics, Jiangxi Provincial People's Hospital, The First Affiliated Hospital of Nanchang Medical College, Nanchang, 330006, Jiangxi, PR China. hanxiaojian@hotmail.com.
  • 6 Institute of Geriatrics, Jiangxi Provincial People's Hospital, The First Affiliated Hospital of Nanchang Medical College, Nanchang, 330006, Jiangxi, PR China. wangxy202201@163.com.
  • # Contributed equally.
PMID: 40473847 DOI: 10.1038/s41698-025-00964-z
Abstract

Glioma is the most common primary malignant brain tumor with high mortality and poor prognosis. Aerobic glycolysis is crucial for the malignant behavior of glioma by promoting their growth. Tripartite motif containing 65 (TRIM65) as an E3 ubiquitin Ligase has been implicated in tumor progression, but its role and regulatory mechanism on aerobic glycolysis in glioma remains unclear. Here, it was demonstrated that TRIM65 was highly expressed in human glioma tissues and associated with poor prognosis. Moreover, TRIM65 knockdown inhibited the glioma cells proliferation in vitro and in vivo. RNA Sequencing and biological verifications were performed to elucidate a novel mechanism underlying TRIM65 silencing attenuated glycolysis and enhanced OXPHOX to suppress the growth of glioma cells. Subsequently, we found that TRIM65 interacted with AMPK, a metabolic sensor, and mediated its K48-linkage ubiquitination and degradation though proteasomal pathway, thereby regulating HIF-1α-induced glycolysis. Importantly, the inhibitory effect of TRIM65 silencing on glycolysis was abrogated by AMPK knockdown or HIF-1α overexpression, indicating glucose metabolic reprogramming by TRIM65 is dependent on AMPK and HIF-1α pathway. These results reveal a new role for TRIM65/AMPK/HIF-1α axis in glioma cell proliferation and aerobic glycolysis, suggesting that TRIM65 may be a potential therapeutic target for intervention of glioma.

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