2. Academic Validation
  3. Dichapetalin-type triterpenoids inhibits macrophage interferon expression by regulating the cGas-STING pathway, suggesting potential application in immunosuppression

Dichapetalin-type triterpenoids inhibits macrophage interferon expression by regulating the cGas-STING pathway, suggesting potential application in immunosuppression

  • Immunopharmacol Immunotoxicol. 2025 Jun 9:1-8. doi: 10.1080/08923973.2025.2513476.
Chuan Zhao 1,  Yi-Ming Sun 1,  Guo-Rong Li 2,  Yue Wang 3,  Yu-Rong Da 2,  Sheng-An Tang 3,  Long Li 2
Affiliations

Affiliations

  • 1 Department of Pharmacology, School of Basic Medical Sciences, Tianjin Medical University, Tianjin, China.
  • 2 Tianjin Institute of Immunology, Key Laboratory of Immune Microenvironment and Disease of the Ministry of Education and Department of Immunology, Tianjin Medical University, Tianjin, China.
  • 3 Tianjin Key Laboratory on Technologies Enabling Development Clinical Therapeutics and Diagnostics (Theranostics), School of Pharmacy, Tianjin Medical University, Tianjin, China.
PMID: 40443028 DOI: 10.1080/08923973.2025.2513476
Abstract

Background: Dichapetalin-type triterpenoids (DTs) derived from Dichapetalum longipetalum (Turcz.) Engl. have attracted extensive attention due to their novel structure, as well as potent anti-tumor and anti-inflammatory activities. In this study, the immunosuppressive effect of Dichapetalin-type triterpenoids on mouse peritoneal macrophages (MPMs) was studied.

Methods: MPMs were stimulated with HSV-1 or LPS for the inflammation model. The cytokines and inflammatory mediators were detected by RT-PCR. Western blotting was carried out to determine the phosphorylation of TBK1 and IRF3.

Results: Our results showed that DTs inhibited the expression of IFN-β in MPMs infected with HSV-1, and also inhibited the expression of Il-1β and IL-6 in LPS-stimulated MPMs. In addition, compound 1 (dichapetalin A) down regulated the phosphorylation of TBK1 and Irf3 in HSV-1-infected MPMs.

Conclusion: Taken together, this study suggests that DTs isolated from the Dichapetalum longipetalum (Turcz.) Engl. inhibits macrophage activation through the cGas-STING pathway in MPMs, which would be potential for the treatment of autoimmune diseases.

Keywords

Dichapetalin-type triterpenoids; IFN-β; autoimmune diseases; cGas-STING pathway; immunosuppression.

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