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  2. Transcriptomic and functional analyses reveal a complex unexplored landscape of Botrytis cinerea colonization in rose

Transcriptomic and functional analyses reveal a complex unexplored landscape of Botrytis cinerea colonization in rose

  • J Exp Bot. 2025 May 17:eraf219. doi: 10.1093/jxb/eraf219.
Rui Li 1 Juanni Yao 1 Jiaying Xiao 1 Yue Ming 1 Daofeng Liu 2 Yueqing Cao 1 Zhensheng Kang 3 Zhengguo Li 1 Yulin Cheng 1
Affiliations

Affiliations

  • 1 Key Laboratory of Plant Hormones and Molecular Breeding of Chongqing, School of Life Sciences, Chongqing University, Chongqing, China.
  • 2 Chongqing Engineering Research Center for Floriculture, Key Laboratory of Horticulture Science for Southern Mountainous Regions of Ministry of Education, College of Horticulture and Landscape Architecture, Southwest University, Chongqing, China.
  • 3 State Key Laboratory for Crop Stress Resistance and High-Efficiency Production, College of Plant Protection, Northwest A&F University, Shaanxi, Yangling, China.
Abstract

Botrytis cinerea (Bc) is a notorious necrotrophic Fungal pathogen that colonizes different plant tissues. Gray mold caused by Bc is a great threat to rose (Rosa sp.), one of the most important ornamental Plants worldwide, but colonization strategies of Bc in rose tissues remain unexplored. Here, we report a comprehensive investigation of the mechanisms underlying Bc colonization in rose leaf and petal by integrated transcriptomic and functional analyses. Multiple genes involved in the biogenesis of ribosome, an organelle for protein synthesis, were commonly upregulated during Bc colonization in leaf and petal. Application of inhibitors targeting Fungal ribosome biogenesis, coupled with gene disruption assays, demonstrated the contribution of ribosome biogenesis to Bc colonization in leaf and petal. Notably, genes associated with nitrogen transport, carbohydrate metabolism, and protein glycosylation contributed to Bc colonization, and some of them were tissue-specific virulence factors. Through in silico secretome analysis and functional verification, we identified seven novel plant cell death-inducing effectors and one of them contributed to Bc colonization in leaf and petal. This study reveals a complex unexplored landscape of Bc colonization in rose and also advances the understanding of plant-pathogen interactions.

Keywords

effector protein; fungal colonization; ornamental plants; transcriptome analysis; virulence factor.

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