2. Academic Validation
  3. Outer membrane vesicles from Pseudomonas aeruginosa induce autophagy-regulated pyroptosis in THP-1 cells

Outer membrane vesicles from Pseudomonas aeruginosa induce autophagy-regulated pyroptosis in THP-1 cells

  • Arch Microbiol. 2025 Feb 10;207(3):54. doi: 10.1007/s00203-025-04264-9.
Jing Ge # 1 2,  Yaoyang Liu # 1 2,  Tianqi Wu 3,  Sitian Yi 4,  Jiang Pu 1,  Juan Gu 5,  Feng Wang 1,  Juan Yu 6 7
Affiliations

Affiliations

  • 1 Department of Laboratory Medicine, Affiliated Hospital of Nantong University, Nantong, 226001, P.R. China.
  • 2 Medical School of Nantong University, Nantong, 226001, P.R. China.
  • 3 Department of Biomedical Engineering, Whiting School of Engineering, Johns Hopkins University, Baltimore, 21218, USA.
  • 4 Institute of Public Health, Nantong University, Nantong, 226019, P.R. China.
  • 5 Qidong Hospital of Traditional Chinese Medicine, Qidong, 226200, P.R. China.
  • 6 Department of Laboratory Medicine, Affiliated Hospital of Nantong University, Nantong, 226001, P.R. China. yujuanjs@163.com.
  • 7 Institute of Public Health, Nantong University, Nantong, 226019, P.R. China. yujuanjs@163.com.
  • # Contributed equally.
PMID: 39928143 DOI: 10.1007/s00203-025-04264-9
Abstract

Outer membrane vesicles derived from Pseudomonas aeruginosa (PA-OMVs) play a crucial role in Bacterial pathogenesis, mediating immune modulation and inflammation. Autophagy, a process that degrades damaged organelles, and Pyroptosis, a form of programmed cell death, both regulate immune responses and contribute to Infection defense. However, the relationship between PA-OMVs, Autophagy, and Pyroptosis remains insufficiently explored, particularly regarding their regulatory mechanisms. This study investigates how PA-OMVs influence cellular Autophagy and Pyroptosis, with the aim of identifying potential therapeutic strategies for infectious diseases. Bulk RNA Sequencing and bioinformatics analysis were conducted on cells treated with PA-OMVs. Autophagy inhibitors, chloroquine (CQ) and 3-methyladenine (3-MA), were used to explore their effects on Pyroptosis, with RT-PCR and ELISA applied to assess Pyroptosis levels. The results revealed a complex interplay between Autophagy and Pyroptosis, with PA-OMVs modulating key immune and inflammatory pathways. Autophagy inhibition decreased the expression of Pyroptosis markers, suggesting a regulatory role. These findings highlight the potential of targeting the autophagy-pyroptosis axis for new Infection control strategies and vaccine development.

Keywords

Pseudomonas aeruginosa; Autophagy; Outer membrane vesicles; Pyroptosis; RNA sequencing.

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