2. Academic Validation
  3. 3-Indoleacetonitrile Is Highly Effective in Treating Influenza A Virus Infection In Vitro and In Vivo

3-Indoleacetonitrile Is Highly Effective in Treating Influenza A Virus Infection In Vitro and In Vivo

  • Viruses. 2021 Jul 23;13(8):1433. doi: 10.3390/v13081433.
Xuejin Zhao 1,  Lianzhong Zhao 2 3 4,  Ya Zhao 2 3 4,  Kun Huang 2 3 4,  Wenxiao Gong 2 3 4,  Ying Yang 2 3 4,  Li Zhao 1,  Xiaohan Xia 1,  Zaiyun Li 5,  Feng Sheng 1,  Xuezhu Du 1,  Meilin Jin 2 3 4
Affiliations

Affiliations

  • 1 State Key Laboratory of Biocatalysis and Enzyme Engineering, School of Life Sciences, Hubei University, Wuhan 430062, China.
  • 2 State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, China.
  • 3 College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China.
  • 4 Key Laboratory of Development of Veterinary Diagnostic Products, Ministry of Agriculture, Wuhan 430070, China.
  • 5 National Key Lab of Crop Genetic Improvement, National Center of Oil Crop Improvement (Wuhan), College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, China.
PMID: 34452298 DOI: 10.3390/v13081433
Abstract

Influenza A viruses are serious zoonotic pathogens that continuously cause pandemics in several animal hosts, including birds, pigs, and humans. Indole derivatives containing an indole core framework have been extensively studied and developed to prevent and/or treat viral Infection. This study evaluated the anti-influenza activity of several indole derivatives, including 3-indoleacetonitrile, indole-3-carboxaldehyde, 3-carboxyindole, and gramine, in A549 and MDCK cells. Among these compounds, 3-indoleacetonitrile exerts profound Antiviral activity against a broad spectrum of influenza A viruses, as tested in A549 cells. Importantly, in a mouse model, 3-indoleacetonitrile with a non-toxic concentration of 20 mg/kg effectively reduced the mortality and weight loss, diminished lung virus titers, and alleviated lung lesions of mice lethally challenged with A/duck/Hubei/WH18/2015 H5N6 and A/Puerto Rico/8/1934 H1N1 influenza A viruses. The Antiviral properties enable the potential use of 3-indoleacetonitrile for the treatment of IAV Infection.

Keywords

3-indoleacetonitrile; antiviral; indole derivatives; indole-3-carboxaldehyde; influenza A virus.

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