2. Academic Validation
  3. Phytantriol-Based Cubosome Formulation as an Antimicrobial against Lipopolysaccharide-Deficient Gram-Negative Bacteria

Phytantriol-Based Cubosome Formulation as an Antimicrobial against Lipopolysaccharide-Deficient Gram-Negative Bacteria

  • ACS Appl Mater Interfaces. 2020 Oct 7;12(40):44485-44498. doi: 10.1021/acsami.0c13309.
Xiangfeng Lai 1,  Yue Ding 1 2,  Chun-Ming Wu 3 4,  Xiaoyu Chen 1,  Jhih-Hang Jiang 5,  Hsien-Yi Hsu 6 7,  Yajun Wang 8,  Anton P Le Brun 3,  Jiangning Song 2,  Mei-Ling Han 5,  Jian Li 5,  Hsin-Hui Shen 1 2
Affiliations

Affiliations

  • 1 Department of Materials Science and Engineering, Faculty of Engineering, Monash University, Clayton, Victoria 3800, Australia.
  • 2 Biomedicine Discovery Institute and Department of Biochemistry and Molecular Biology, Monash University, Clayton, Victoria 3800, Australia.
  • 3 Australian Centre for Neutron Scattering, Australian Nuclear Science and Technology Organisation, Locked Bag 2001, Kirrawee DC, New South Wales 2232, Australia.
  • 4 National Synchrotron Radiation Research Center, Hsinchu 30076, Taiwan.
  • 5 Infection and Immunity Program, Monash Biomedicine Discovery Institute and Department of Microbiology, Monash University, Clayton, Victoria 3800, Australia.
  • 6 School of Energy and Environment & Department of Materials Science and Engineering, City University of Hong Kong, Kowloon Tong, Hong Kong, China.
  • 7 Shenzhen Research Institute of City University of Hong Kong, Shenzhen 518057, China.
  • 8 College of Chemistry & Materials Engineering, Wenzhou University, Wenzhou 325027, Zhejiang, China.
PMID: 32942850 DOI: 10.1021/acsami.0c13309
Abstract

Treatment of multidrug-resistant (MDR) Bacterial infections increasingly relies on last-line Antibiotics, such as polymyxins, with the urgent need for discovery of new antimicrobials. Nanotechnology-based antimicrobials have gained significant importance to prevent the catastrophic emergence of MDR over the past decade. In this study, phytantriol-based nanoparticles, named cubosomes, were prepared and examined in vitro by minimum inhibitory concentration (MIC) and time-kill assays against Gram-negative bacteria: Acinetobacter baumannii, Klebsiella pneumoniae, and Pseudomonas aeruginosa. Phytantriol-based cubosomes were highly bactericidal against polymyxin-resistant, lipopolysaccharide (LPS)-deficient A. baumannii strains. Small-angle neutron scattering (SANS) was employed to understand the structural changes in biomimetic membranes that replicate the composition of these LPS-deficient strains upon treatment with cubosomes. Additionally, to further understand the membrane-cubosome interface, neutron reflectivity (NR) was used to investigate the interaction of cubosomes with model Bacterial membranes on a solid support. These results reveal that cubosomes might be a new strategy for combating LPS-deficient Gram-negative pathogens.

Keywords

antimicrobials; cubosomes; lipopolysaccharide-deficient; multidrug resistance; outer membrane.

Figures
Products