2. Academic Validation
  3. Natural Tr1-like cells do not confer long-term tolerogenic memory

Natural Tr1-like cells do not confer long-term tolerogenic memory

  • Elife. 2019 Oct 11:8:e44821. doi: 10.7554/eLife.44821.
Koshika Yadava # 1 2,  Carlos Obed Medina # 1,  Heather Ishak 1,  Irina Gurevich 1,  Hedwich Kuipers 1 3,  Elya Ali Shamskhou 1,  Ievgen O Koliesnik 1,  James J Moon 4 5,  Casey Weaver 6,  Kari Christine Nadeau 7,  Paul L Bollyky 1
Affiliations

Affiliations

  • 1 Division of Infectious Diseases and Geographic Medicine, Department of Medicine, Beckman Center, Stanford University School of Medicine, Stanford, United States.
  • 2 Radcliffe Department of Medicine, Weatherall Institute of Molecular Medicine, University of Oxford, Oxford, United Kingdom.
  • 3 Department of Clinical Neurosciences, University of Calgary, Calgary, Canada.
  • 4 Center for Immunology and Inflammatory Diseases, Massachusetts General Hospital, Harvard Medical School, Charlestown, United States.
  • 5 Division of Pulmonary and Critical Care Medicine, Massachusetts General Hospital, Harvard Medical School, Charlestown, United States.
  • 6 Bevill Biomedical Research Building, The University of Alabama at Birmingham, Birmingham, United States.
  • 7 Sean N Parker Center for Allergy & Asthma Research, Stanford University, Mountain View, United States.
  • # Contributed equally.
PMID: 31603425 DOI: 10.7554/eLife.44821
Abstract

IL-10-producing Tr1 cells promote tolerance but their contributions to tolerogenic memory are unclear. Using 10BiT mice that carry a Foxp3-eGFP reporter and stably express CD90.1 following IL-10 production, we characterized the spatiotemporal dynamics of Tr1 cells in a house dust Mite model of allergic airway inflammation. CD90.1+Foxp3-IL-10+ Tr1 cells arise from memory cells and rejoin the tissue-resident memory T-cell pool after cessation of IL-10 production. Persistent antigenic stimulation is necessary to sustain IL-10 production and Irf1 and Batf expression distinguishes CD90.1+Foxp3-IL-10+ Tr1 cells from CD90.1+Foxp3-IL-10- 'former' Tr1. Depletion of Tr1-like cells after primary sensitization exacerbates allergic airway inflammation. However, neither transfer nor depletion of former Tr1 cells influences either Tr1 numbers or the inflammatory response during subsequent allergen memory re-challenge weeks later. Together these data suggest that naturally-arising Tr1 cells do not necessarily give rise to more Tr1 upon allergen re-challenge or contribute to tolerogenic memory. This phenotypic instability may limit efforts to re-establish tolerance by expanding Tr1 in vivo.

Keywords

IL-10; TR1; allergy; house dust mite; immunology; inflammation; memory; mouse.

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