2. Academic Validation
  3. AtTrm5a catalyses 1-methylguanosine and 1-methylinosine formation on tRNAs and is important for vegetative and reproductive growth in Arabidopsis thaliana

AtTrm5a catalyses 1-methylguanosine and 1-methylinosine formation on tRNAs and is important for vegetative and reproductive growth in Arabidopsis thaliana

  • Nucleic Acids Res. 2019 Jan 25;47(2):883-898. doi: 10.1093/nar/gky1205.
Xiaohuan Jin 1 2,  Zhengyi Lv 1 2,  Junbao Gao 1 2,  Rui Zhang 1 2,  Ting Zheng 3 4,  Ping Yin 3 4,  Dongqin Li 4,  Liangcai Peng 1 2,  Xintao Cao 5,  Yan Qin 5,  Staffan Persson 6 7,  Bo Zheng 8,  Peng Chen 1 2
Affiliations

Affiliations

  • 1 College of Plant Science and Technology, HuaZhong Agricultural University, Wuhan 430070, China.
  • 2 Biomass and Bioenergy Research Centre, HuaZhong Agricultural University, Wuhan 430070, China.
  • 3 College of Life Science, HuaZhong Agricultural University, Wuhan 430070, China.
  • 4 National Key Laboratory of Crop Genetic Improvement, HuaZhong Agricultural University, Wuhan 430070, China.
  • 5 Institute of Biophysics, Chinese Academy of Sciences, China.
  • 6 School of Biosciences, University of Melbourne, Parkville 3010, VIC, Australia.
  • 7 Joint International Research Laboratory of Metabolic & Developmental Sciences, Shanghai Jiao Tong University-University of Adelaide Joint Centre for Agriculture and Health, State Key Laboratory of Hybrid Rice, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China.
  • 8 College of Horticulture and Forestry Sciences, HuaZhong Agricultural University, Wuhan 430070, China.
PMID: 30508117 DOI: 10.1093/nar/gky1205
Abstract

Modified nucleosides on tRNA are critical for decoding processes and protein translation. tRNAs can be modified through 1-methylguanosine (m1G) on position 37; a function mediated by Trm5 homologs. We show that AtTRM5a (At3g56120) is a Trm5 ortholog in Arabidopsis thaliana. AtTrm5a is localized to the nucleus and its function for m1G and m1I methylation was confirmed by mutant analysis, yeast complementation, m1G nucleoside level on single tRNA, and tRNA in vitro methylation. Arabidopsis attrm5a mutants were dwarfed and had short filaments, which led to reduced seed setting. Proteomics data indicated differences in the abundance of proteins involved in photosynthesis, ribosome biogenesis, Oxidative Phosphorylation and calcium signalling. Levels of Phytohormone auxin and jasmonate were reduced in attrm5a mutant, as well as expression levels of genes involved in flowering, shoot apex cell fate determination, and hormone synthesis and signalling. Taken together, loss-of-function of AtTrm5a impaired m1G and m1I methylation and led to aberrant protein translation, disturbed hormone homeostasis and developmental defects in Arabidopsis Plants.

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