2. Academic Validation
  3. Neuroprotective Effect of Hydroxytyrosol in Experimental Diabetes Mellitus

Neuroprotective Effect of Hydroxytyrosol in Experimental Diabetes Mellitus

  • J Agric Food Chem. 2017 Jun 7;65(22):4378-4383. doi: 10.1021/acs.jafc.6b02945.
José Julio Reyes 1,  Beatriz Villanueva 2,  Juan Antonio López-Villodres 1,  José Pedro De La Cruz 1,  Lidia Romero 1,  María Dolores Rodríguez-Pérez 1,  Guillermo Rodriguez-Gutierrez 3,  Juan Fernández-Bolaños 3,  José Antonio González-Correa 1
Affiliations

Affiliations

  • 1 Departmento de Farmacologı́a, Facultad de Medicina, Instituto de Investigación Biomédica (IBIMA), Universidad de Málaga , Campus de Teatinos s/n, 29071Málaga, Spain.
  • 2 Universidad Metropolitana de Puerto Rico (UMET) , San Juan PR 00926-2602, Puerto Rico.
  • 3 Instituto de la Grasa, Consejo Superior de Investigaciones Cientı́ficas (CSIC) , Campus Universitario Pablo de Olavide, Ctra. Utrera Km 1, Edificio 46, 41013 Seville, Spain.
PMID: 27700078 DOI: 10.1021/acs.jafc.6b02945
Abstract

The aim of the study was to analyze the possible neuroprotective effect of hydroxytyrosol (HT) in diabetic Animals in a model of hypoxia-reoxygenation. Rats (10 Animals/group) were distributed in five groups: nondiabetic rats, control diabetic rats (DR), and DR rats treated for 2 months with 1, 5, or 10 mg/kg/day po HT. At the end of follow-up, an experimental model of hypoxia-reoxygenation in brain slices was tested. The DR group showed increased cell death, oxidative and nitrosative stress, and an increase in brain inflammatory mediators. These alterations were significantly greater in DR than in normoglycemic Animals. HT significantly reduced oxidative (38.5-52.4% lipid peroxidation) and nitrosative stress (48.0-51.0% nitric oxide and 43.9-75.2% peroxynitrite concentration) and brain inflammatory mediators (18.6-40.6% prostaglandin E2 and 17.0-65.0% interleukin 1β concentration). Cell death was reduced by 25.9, 37.5, and 41.0% after the administration of 1, 5, or 10 mg/kg/day. The administration of HT in rats with experimental diabetes thus had a neuroprotective effect.

Keywords

diabetes mellitus; hydroxytyrosol; inflammatory mediators; neuroprotection; oxidative stress.

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