2. Academic Validation
  3. Comparative investigation of the binding characteristics of poly-L-lysine and chitosan on alginate hydrogel

Comparative investigation of the binding characteristics of poly-L-lysine and chitosan on alginate hydrogel

  • Int J Biol Macromol. 2016 Mar:84:135-41. doi: 10.1016/j.ijbiomac.2015.12.008.
Ying Ren 1,  Hongguo Xie 2,  Xiaocen Liu 1,  Jie Bao 3,  Weiting Yu 4,  Xiaojun Ma 5
Affiliations

Affiliations

  • 1 Laboratory of Biomedical Material Engineering, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China; University of Chinese Academy of Sciences, Beijing 100049, China.
  • 2 Laboratory of Biomedical Material Engineering, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China. Electronic address: xiehg@dicp.ac.cn.
  • 3 State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China.
  • 4 Laboratory of Biomedical Material Engineering, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China. Electronic address: yuwt@dicp.ac.cn.
  • 5 Laboratory of Biomedical Material Engineering, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China.
PMID: 26683880 DOI: 10.1016/j.ijbiomac.2015.12.008
Abstract

The binding properties of poly-L-lysine and chitosan to alginate have been evaluated quantitatively and compared. Poly-L-lysine bound to alginate hydrogel more rapidly than chitosan as poly-L-lysine has a smaller molar hydrodynamic volume. In addition, poly-L-lysine showed a much higher binding capacity (6.14:1) for alginate hydrogel beads than chitosan (2.71:1), and a little higher binding stoichiometry (0.58) to sodium alginate molecules in solution than chitosan (0.49). An exothermic heat of alginate-poly-L-lysine complexes formation of 2.02 kJ/mol was detected. For alginate-chitosan complexes, the binding enthalpy has been seen to be -3.49 kJ/mol. The stability of the polyelectrolyte complexes was related to their binding enthalpy. The alginate-poly-L-lysine complexes could be disintegrated and rebuilt. By contrast, chitosan was bound with alginate in a steady state. These results provide fundamental insights regarding the structure and property relationships of macromolecules, and will be helpful in designing and selecting appropriate Polymers.

Keywords

Alginate; Chitosan; Poly-l-lysine.

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