2. Academic Validation
  3. Effects of isoprothiolane on cell growth of cultured bovine mammary epithelial cells

Effects of isoprothiolane on cell growth of cultured bovine mammary epithelial cells

  • J Vet Med Sci. 1999 May;61(5):553-6. doi: 10.1292/jvms.61.553.
H Okada 1,  Y Miyake,  H Ohtsuka,  Y Kiku,  S Fukuda,  A Watanabe,  Y Yokomizo,  T J Rosol,  T Yoshino
Affiliations

Affiliation

  • 1 Department of Veterinary Pathology, Rakuno Gakuen University, Ebetsu, Hokkaido, Japan.
PMID: 10379950 DOI: 10.1292/jvms.61.553
Abstract

This study was performed to investigate the effects of isoprothiolane on cell growth and the production of interleukin (IL)-1 and IL-6 by bovine mammary epithelial cells in vitro. Isoprothiolane increased proliferation of mammary epithelial cells in a dose-dependent manner at the concentration of 0.05 to 5 microM when cultured either with or without serum-supplemented medium. In contrast, isoprothiolane (0.0005-5 microM) significantly inhibited the production of IL-1 and IL-6 by mammary epithelial cells. Moreover, the cytokines, IL-1alpha, IL-1beta, IL-6, and tumor necrosis factor (TNF)-alpha tended to inhibit the proliferation of mammary epithelial cells in a dose-dependent manner. These results indicated that isoprothiolane regulated mammary epithelial cell growth in vitro possibly by modulating the production of cytokines.

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