2. Dye Reagents

Dye Reagents

A dye is a colored substance that has an affinity to the substrate to which it is being applied.

A new approach is presented for analysis of microplastics in environmental samples, based on selective fluorescent staining using Nile Red (NR), followed by density-based extraction and filtration. The dye adsorbs onto plastic surfaces and renders them fluorescent when irradiated with blue light. Fluorescence emission is detected using simple photography through an orange filter. Image-analysis allows fluorescent particles to be identified and counted. Magnified images can be recorded and tiled to cover the whole filter area, allowing particles down to a few micrometres to be detected. The solvatochromic nature of Nile Red also offers the possibility of plastic categorisation based on surface polarity characteristics of identified particles. This article details the development of this staining method and its initial cross-validation by comparison with infrared (IR) microscopy. Microplastics of different sizes could be detected and counted in marine sediment samples. The fluorescence staining identified the same particles as those found by scanning a filter area with IR-microscopy.

Cyanine dyes can be used to quantify the amount of dsDNA within a sample. The linearity of fluorescence, as function of DNA amount of six dyes, is obtained by measuring the fluorescence intensity at the optimal excitation and emission maxima.

Dye Reagents (3120):

Cat. No. Product Name CAS No. Purity Chemical Structure
  • HY-D0896
    ANS 82-76-8 98.0%
    ANS (8-Anilino-1-naphthalenesulfonic acid) is a competitive inhibitor targeting thyroxine binding globulin (TBG) (Ki=2.09×106 M-1). ANS is used in radioimmunoassay by displacing bound triiodothyronine (T3) to improve detection sensitivity. ANS can block the protein binding site of T3 and release free T3 for antibody recognition. As a fluorescent probe, ANS can specifically bind to the hydrophobic region of proteins (such as membrane proteins) and monitor the dynamics of protein conformation through changes in fluorescent signals. It is widely used in biochemical research and antibacterial material development.
    ANS
  • HY-D0014
    Brilliant blue G-250 6104-58-1
    Brilliant Blue G-250 is a dye commonly used for the visualization of proteins separated by SDS-PAGE, offering a simple staining procedure and high quantitation. In the Bradford protein assay, protein concentrations are determined by the absorbance at 595 nm due to the binding of Brilliant Blue G-250 to proteins. Brilliant Blue G-250 is a safe highly selective P2×7R antagonist with promising consequent inactivation of NLRP3 inflammasome.
    Brilliant blue G-250
  • HY-W127711
    Fast Blue B Salt, Dye content ~95% 14263-94-6
    Fast Blue B Salt, Dye content ~95% is a diazo dye, primarily used as a chromogenic substrate in biochemical analysis. Fast Blue B Salt, Dye content ~95% is the coupling agent for the histochemical demonstration of proteases. Fast Blue B Salt, Dye content ~95% can be used for the detection of acid phosphatase in Clostridium perfringens, and also for the determination of lipase activity. Fast Blue B Salt, Dye content ~95% reacts directly with the active hydroxyl groups in phenolic compounds to determine the content of phenolic substances.
    Fast Blue B Salt, Dye content ~95%
  • HY-D0896A
    ANS ammonium 28836-03-5 99.94%
    ANS ammonium is a potent antibacterial agent and a textile dye. ANS ammonium can be used as fluorescence probe. ANS ammonium blocks the binding of triiodothyronine to thyroxine binding globulin in radioimmunoassay of triiodothyronine.
    ANS ammonium
  • HY-D0800
    Mordant Blue 29 1667-99-8
    Chromeazurol S is a compound belonging to the class of azo dyes. It is often used as an indicator in analytical chemistry to detect metal ions such as copper, nickel, and cobalt. Chromeazurol S turns from yellow to blue in the presence of metal ions, allowing them to be detected and quantified. It can be applied to a test strip or added directly to a solution for analysis.
    Mordant Blue 29
  • HY-D0015
    Cresol red, 97% 1733-12-6 99.30%
    Cresol red, 97% is an anionic dye used as a pH indicator (pH 1.8 orange - pH 2.0 yellow, pH 7.0 yellow - pH 8.8 purple).
    Cresol red, 97%
  • HY-D0265
    Thymolphthalein 125-20-2 99.85%
    Thymolphthalein is an electrochemically active dye due to the presence of thymol fragments. Thymolphthalein is a Phthalein dye used as an acid-base indicator. It is colourless in acid pH and is blue in basic pH.
    Thymolphthalein
  • HY-D0304
    Rhodamine B base 509-34-2
    Rhodamine B base (Solvent red 49) is a fluorescent dye (Ex=543 nM; Em=565 nM).
    Rhodamine B base
  • HY-W353607A
    Bromochlorophenol Blue sodium salt 102185-52-4 98%
    Bromochlorophenol Blue sodium salt, an acid-sensitive dye, is a pH-indicating dye.
    Bromochlorophenol Blue sodium salt
  • HY-D0896AR
    ANS ammonium (Standard) 28836-03-5
    ANS (ammonium) (Standard) is the analytical standard of ANS (ammonium). This product is intended for research and analytical applications. ANS ammonium is a potent antibacterial agent and a textile dye. ANS ammonium can be used as fluorescence probe. ANS ammonium blocks the binding of triiodothyronine to thyroxine binding globulin in radioimmunoassay of triiodothyronine.
    ANS ammonium (Standard)