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  3. Rhodamine B base

Rhodamine B base  (Synonyms: Solvent red 49)

Cat. No.: HY-D0304 Purity: 97.0%
Handling Instructions Technical Support

Rhodamine B base (Solvent red 49) is a fluorescent dye (Ex=543 nM; Em=565 nM).

For research use only. We do not sell to patients.

Rhodamine B base

Rhodamine B base Chemical Structure

CAS No. : 509-34-2

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Description

Rhodamine B base (Solvent red 49) is a fluorescent dye (Ex=543 nM; Em=565 nM)[1].

Molecular Weight

442.55

Formula

C28H30N2O3

CAS No.
Appearance

Solid

Color

Purple to purplish red

SMILES

O=C1OC2(C3=C1C=CC=C3)C4=CC=C(N(CC)CC)C=C4OC5=CC(N(CC)CC)=CC=C52

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : 40 mg/mL (90.39 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.2596 mL 11.2982 mL 22.5963 mL
5 mM 0.4519 mL 2.2596 mL 4.5193 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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Dosing volume
(per animal)

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Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.2596 mL 11.2982 mL 22.5963 mL 56.4908 mL
5 mM 0.4519 mL 2.2596 mL 4.5193 mL 11.2982 mL
10 mM 0.2260 mL 1.1298 mL 2.2596 mL 5.6491 mL
15 mM 0.1506 mL 0.7532 mL 1.5064 mL 3.7661 mL
20 mM 0.1130 mL 0.5649 mL 1.1298 mL 2.8245 mL
25 mM 0.0904 mL 0.4519 mL 0.9039 mL 2.2596 mL
30 mM 0.0753 mL 0.3766 mL 0.7532 mL 1.8830 mL
40 mM 0.0565 mL 0.2825 mL 0.5649 mL 1.4123 mL
50 mM 0.0452 mL 0.2260 mL 0.4519 mL 1.1298 mL
60 mM 0.0377 mL 0.1883 mL 0.3766 mL 0.9415 mL
80 mM 0.0282 mL 0.1412 mL 0.2825 mL 0.7061 mL
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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Rhodamine B base
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