L-Leucyl-4-nitroanilide hydrochloride

Name: L-Leucyl-4-nitroanilide hydrochloride
Brand: MedChemExpress
SKU: HY-137872
Rating: 4.5 (1 reviews)

Cat. No.: HY-137872 Purity: 99.97%: Data Sheet
COA

SDS
Handling Instructions
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L-Leucyl-4-nitroanilide hydrochloride is a commonly used substrate in various biochemical assays to measure the activity of enzymes that cleave peptide bonds, such as proteases. L-Leucine p-nitroanilide hydrochloride has unique chemical properties that allow it to be cleaved by these enzymes to form a yellow product called p-nitroaniline. This makes it a useful tool for detecting and quantifying protease activity in biological samples such as blood or tissue extracts.

For research use only. We do not sell to patients.

L-Leucyl-4-nitroanilide hydrochloride Chemical Structure

CAS No. : 16010-98-3

Size	Stock	Quantity
250 mg	In-stock	Estimated Time of Arrival: December 31
1 g	In-stock	Estimated Time of Arrival: December 31
5 g	Get quote
10 g	Get quote

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Based on 1 publication(s) in Google Scholar

Other Forms of L-Leucyl-4-nitroanilide hydrochloride:

L-Leucyl-4-nitroanilide In-stock

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Biological Activity
Purity & Documentation
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Description

In Vitro

L-Leucine p-nitroanilide (hydrochloride) is a biochemical reagent that can be used as a biological material or organic compound for life science related research.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

287.74

Formula

C₁₂H₁₈ClN₃O₃

CAS No.

16010-98-3

Appearance

Solid

Color

Off-white to light yellow

SMILES

N[C@@H](CC(C)C)C(NC1=CC=C([N+]([O-])=O)C=C1)=O.Cl

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (347.54 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.4754 mL	17.3768 mL	34.7536 mL
5 mM	0.6951 mL	3.4754 mL	6.9507 mL
10 mM	0.3475 mL	1.7377 mL	3.4754 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

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(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

Purity & Documentation

Purity: 99.97%

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Data Sheet (242 KB) SDS (393 KB)

COA (246 KB)

Handling Instructions (2659 KB)

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	3.4754 mL	17.3768 mL	34.7536 mL	86.8840 mL
	5 mM	0.6951 mL	3.4754 mL	6.9507 mL	17.3768 mL
	10 mM	0.3475 mL	1.7377 mL	3.4754 mL	8.6884 mL
	15 mM	0.2317 mL	1.1585 mL	2.3169 mL	5.7923 mL
	20 mM	0.1738 mL	0.8688 mL	1.7377 mL	4.3442 mL
	25 mM	0.1390 mL	0.6951 mL	1.3901 mL	3.4754 mL
	30 mM	0.1158 mL	0.5792 mL	1.1585 mL	2.8961 mL
	40 mM	0.0869 mL	0.4344 mL	0.8688 mL	2.1721 mL
	50 mM	0.0695 mL	0.3475 mL	0.6951 mL	1.7377 mL
	60 mM	0.0579 mL	0.2896 mL	0.5792 mL	1.4481 mL
	80 mM	0.0434 mL	0.2172 mL	0.4344 mL	1.0860 mL
	100 mM	0.0348 mL	0.1738 mL	0.3475 mL	0.8688 mL