2. Isotope-Labeled Compounds
  3. Research Area
  4. SILAC Reagents

SILAC Reagents

Stable isotope labeling by amino acids in cell culture (SILAC) is the most commonly used method for quantitative proteomic analysis, thanks to its simplicity and high reproducibility. It was first used in quantitative proteomics by Ong et al. from the Mann Laboratory in Denmark in 2022. It can provide accurate relative quantification without any chemical derivatization or other operations, and is widely used in research on cell biology, biochemistry, pharmacology, etc [1][2].

Workflow for SILAC experiments

 

Figure 1. Workflow for SILAC

 

References:

[1] Nat Protoc. 2006; 1(6):2650-60.

[2] Proteomics. 2015 Sep; 15(18):3175-92.

SILAC Reagents (537):

Cat. No. Product Name CAS No. Purity Chemical Structure
  • HY-N0623S
    L-Tryptophan-d5 62595-11-3 99.94%
    L-Tryptophan-d5 is the deuterium labeled L-Tryptophan. L-Tryptophan (Tryptophan) is an essential amino acid that is the precursor of serotonin, melatonin, and vitamin B3.
    L-Tryptophan-d<sub>5</sub>
  • HY-N0215S12
    L-Phenylalanine-d5 56253-90-8 99.90%
    L-Phenylalanine-d5 is the deuterium labeled L-Phenylalanine. L-Phenylalanine ((S)-2-Amino-3-phenylpropionic acid) is an essential amino acid isolated from Escherichia coli. L-Phenylalanine is a α2δ subunit of voltage-dependent Ca+ channels antagonist with a Ki of 980 nM. L-phenylalanine is a competitive antagonist for the glycine- and glutamate-binding sites of N-methyl-D-aspartate receptors (NMDARs) (KB of 573 μM ) and non-NMDARs, respectively. L-Phenylalanine is widely used in the production of food flavors and pharmaceuticals.
    L-Phenylalanine-d<sub>5</sub>
  • HY-N0455AS8
    L-Arginine-13C6,15N4 hydrochloride 202468-25-5 99.85%
    L-Arginine-13C6,15N4 (hydrochloride) is the 13C- and 15N-labeled L-Arginine hydrochloride. L-Arginine hydrochloride ((S)-(+)-Arginine hydrochloride) is the nitrogen donor for synthesis of nitric oxide, a potent vasodilator that is deficient during times of sickle cell crisis.
    L-Arginine-<sup>13</sup>C<sub>6</sub>,<sup>15</sup>N<sub>4</sub> hydrochloride
  • HY-W010347S
    DL-Homocysteine-d4 416845-90-4 98.4%
    DL-Homocysteine-d4 is the deuterium labeled DL-Homocysteine. DL-Homocysteine is a weak neurotoxin, and can affect the production of kynurenic acid in the brain.
    DL-Homocysteine-d<sub>4</sub>
  • HY-N0067S
    γ-Aminobutyric acid-d6 70607-85-1 99.92%
    γ-Aminobutyric acid-d6 is the deuterium labeled γ-Aminobutyric acid. γ-Aminobutyric acid (4-Aminobutyric acid) is a major inhibitory neurotransmitter in the adult mammalian brain, binding to the ionotropic GABA receptors (GABAA receptors) and metabotropic receptors (GABAB receptors).
    γ-Aminobutyric acid-d<sub>6</sub>
  • HY-N0486S1
    L-Leucine-13C 74292-94-7 99.4%
    L-Leucine-13C is the 13C-labeled L-Leucine. L-Leucine is an essential branched-chain amino acid (BCAA), which activates the mTOR signaling pathway.
    L-Leucine-<sup>13</sup>C
  • HY-N0486S9
    L-Leucine-d3 87828-86-2 99.93%
    L-Leucine-d3 is the deuterium labeled L-Leucine. L-Leucine is an essential branched-chain amino acid (BCAA), which activates the mTOR signaling pathway.
    L-Leucine-d<sub>3</sub>
  • HY-N0390S8
    L-Glutamine-15N2 204451-48-9 99.10%
    L-Glutamine-15N2 is the 15N-labeled L-Glutamine. L-Glutamine (L-Glutamic acid 5-amide) is a non-essential amino acid present abundantly throughout the body and involved in many metabolic processes. L-Glutamine provides a source of carbons for oxidation in some cells.
    L-Glutamine-<sup>15</sup>N<sub>2</sub>
  • HY-N0650S
    L-Serine-13C3 201595-68-8 98.9%
    L-Serine-13C3 is the 13C-labeled L-Serine. L-Serine ((-)-Serine; (S)-Serine), one of the so-called non-essential amino acids, plays a central role in cellular proliferation.
    L-Serine-<sup>13</sup>C<sub>3</sub>
  • HY-N0455AS6
    L-Arginine-13C6 hydrochloride 201740-91-2 99.00%
    L-Arginine-13C6 (hydrochloride) is the 13C-labeled L-Arginine hydrochloride. L-Arginine hydrochloride ((S)-(+)-Arginine hydrochloride) is the nitrogen donor for synthesis of nitric oxide, a potent vasodilator that is deficient during times of sickle cell crisis.
    L-Arginine-<sup>13</sup>C<sub>6</sub> hydrochloride
  • HY-I1124
    L-Valine-d8 35045-72-8 99.84%
    L-Valine-d8 is a deuterated form of L-Valine. L-Valine-d8 can be used in the labelled synthesis of L-valineamide-d8 intermediate. L-Valine is one of 20 proteinogenic amino acids. L-Valine is an essential amino acid.
    L-Valine-d<sub>8</sub>
  • HY-N0326S5
    L-Methionine-13C5 202326-57-6 99.4%
    L-Methionine-13C5 is the 13C-labeled L-Methionine. L-Methionine is the L-isomer of Methionine, an essential amino acid for human development. Methionine acts as a hepatoprotectant.
    L-Methionine-<sup>13</sup>C<sub>5</sub>
  • HY-N0229S3
    L-Alanine-d4 18806-29-6 99.97%
    L-Alanine-d4 is the deuterium labeled L-Alanine. L-Alanine is a non-essential amino acid, involved in sugar and acid metabolism, increases immunity, and provides energy for muscle tissue, brain, and central nervous system.
    L-Alanine-d<sub>4</sub>
  • HY-Y0252S
    L-Proline-13C5 201740-83-2 99.00%
    L-Proline-13C5 is the 13C-labeled L-Proline. L-Proline is one of the twenty amino acids used in living organisms as the building blocks of proteins.
    L-Proline-<sup>13</sup>C<sub>5</sub>
  • HY-N0717S6
    L-Valine-13C5 55443-52-2 99.6%
    L-Valine-13C5 ((S)-Valine-13C5) is the 13C-labeled L-Valine (HY-N0717). L-Valine ((S)-Valine) is a nonlinear semiorganic material. L-Valine causes lipid peroxidation and accumulation of malondialdehyde (MDA), exhibits inhibitory activity against cyanobacteria. L-Valine inhibits multidrug-resistant bacteria through activation of PI3K/Akt signaling pathway and inhibition of arginase.
    L-Valine-<sup>13</sup>C<sub>5</sub>
  • HY-N0623S2
    L-Tryptophan-13C11 202114-65-6 99.60%
    L-Tryptophan-13C11 is the 13C-labeled L-Tryptophan. L-Tryptophan (Tryptophan) is an essential amino acid that is the precursor of serotonin, melatonin, and vitamin B3.
    L-Tryptophan-<sup>13</sup>C<sub>11</sub>
  • HY-W017018S4
    L-Ornithine-d7 hydrochloride 2483831-57-6 99.90%
    L-Ornithine-d7 (hydrochloride) is the deuterium labeled L-Ornithine hydrochloride. L-Ornithine hydrochloride is a free amino acid that plays a central role in the urea cycle and is also important for the disposal of excess nitrogen.
    L-Ornithine-d<sub>7</sub> hydrochloride
  • HY-W017462S
    Creatine-d5 monohydrate 99.93%
    Creatine-d5 (monohydrate) is the deuterium labeled Creatine monohydrate. Creatine monohydrate, an endogenous amino acid derivative, plays an important role in cellular energy, especially in muscle and brain.
    Creatine-d<sub>5</sub> monohydrate
  • HY-N0394S1
    L-Cystine-d4 1192736-38-1 ≥98.0%
    L-Cystine-d4 is the deuterium labeled L-Cystine. L-Cystine is an amino acid and intracellular thiol, which plays a critical role in the regulation of cellular processes.
    L-Cystine-d<sub>4</sub>
  • HY-N0326S7
    L-Methionine-d3 13010-53-2 99.90%
    L-Methionine-d3 is the deuterium labeled L-Methionine. L-Methionine is the L-isomer of Methionine, an essential amino acid for human development. Methionine acts as a hepatoprotectant.
    L-Methionine-d<sub>3</sub>