Isorhamnetin 3-O-galactoside  (Synonyms: Cacticin)

Isorhamnetin 3-O-galactoside (Cacticin) is a flavonoid glycoside that can be isolated from Oenanthe javanica, with antithrombotic and profibrinolytic activities. Isorhamnetin 3-O-galactoside inhibits the activities of thrombin and factor Xa (FXa) and reduces thrombin-catalyzed fibrin polymerization maximum rate. Isorhamnetin 3-O-galactoside suppresses TNF-α-induced PAI-1 secretion, decreases the PAI-1/tissue-type plasminogen activator (t-PA) ratio, and inhibits FXa production and FVa/FXa-mediated thrombin generation. Isorhamnetin 3-O-galactoside exerts protective effects against Carbon tetrachloride (CCl4) (HY-Y0298)-induced hepatic injury in mice. Isorhamnetin 3-O-galactoside can be used for the study of liver injury-related diseases and thrombotic vascular diseases^[1][2][3].

Isorhamnetin 3-O-galactoside (0.5-50 μM) inhibits TNF-α/FVIIa-mediated thrombin and FXa production in HUVECs^[2].
Isorhamnetin 3-O-galactoside (0.5-50 μM, 18 h) dose-dependently inhibits TNF-α-induced PAI-1 secretion in HUVECs^[2].
Isorhamnetin 3-O-galactoside (1-5 μM, 6 h) potently inhibits LPS (100 ng/ml, 16 h)-induced HMGB1 release in HUVECs^[3].
Isorhamnetin 3-O-galactoside (1-5 μM, 6 h) decreases LPS- or HMGB1-induced barrier disruption ^[3].
Isorhamnetin 3-O-galactoside (5 μM, 6 h) inhibits HMGB1-induced paracellular gap formation in HUVECs and promotes the formation of dense F-actin rings, maintaining the morphological integrity of endothelial cells^[3].
Isorhamnetin 3-O-galactoside (1-5 μM, 6 h) suppresses HMGB1-induced expression of vascular cell adhesion molecule-1 (VCAM-1) in HUVECs^[3].
Isorhamnetin-3-O-galactoside (1-5 μM, 6 h) significantly suppresses HMGB1-induced activation of Phospho-NF-κB p65 and TNF-α in HUVEC nuclear lysates^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Isorhamnetin 3-O-galactoside (50-200 mg/kg, i.p., twice: 30 min before and 2 h after CCl4 injection) attenuates CCl4-induced hepatic injury in male ICR mice^[1].
Isorhamnetin 3-O-galactoside (2.4 mg/kg, intravenous administration, once) significantly prolongs the tail bleeding time^[2].
Isorhamnetin 3-O-galactoside (4.8 mg/mouse, i.v., once) markedly inhibits HMGB1 release in cecal ligation and puncture (CLP)-induced septic male C57BL/6 mice^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

478.40

C₂₂H₂₂O₁₂

6743-92-6

Solid

White to yellow

O=C1C(O[C@H]2[C@@H]([C@H]([C@H]([C@@H](CO)O2)O)O)O)=C(C3=CC=C(O)C(OC)=C3)OC4=CC(O)=CC(O)=C14

Room temperature in continental US; may vary elsewhere.

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

• [1]. Kim DW, et al. Isorhamnetin-3-O-galactoside Protects against CCl4-Induced Hepatic Injury in Mice. Biomol Ther (Seoul). 2012 Jul;20(4):406-12.  [Content Brief]

  [2]. Ku SK, et al. Antithrombotic and profibrinolytic activities of isorhamnetin-3-O-galactoside and hyperoside. Food Chem Toxicol. 2013 Mar;53:197-204.  [Content Brief]

  [3]. Kim TH, et al. Anti-inflammatory activities of isorhamnetin-3-O-galactoside against HMGB1-induced inflammatory responses in both HUVECs and CLP-induced septic mice. J Cell Biochem. 2013 Feb;114(2):336-45.  [Content Brief]