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  5. Cell Transfection
  6. Protein Transfection Reagent

Protein Transfection Reagent 

Cat. No.: HY-K2016
Manual COA SDS Technical Support

MCE Protein Transfection Reagent is a cationic lipid mixture for complexation with proteins, peptides, antibodies and other biologically active molecules to allow their direct intracellular delivery.

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300 μL In-stock
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    MedChemExpress Validation
    Experimental conditions:
    Cell density: 90% density during cell transfectionDosage: 10 μg of EGFP protein (1 mg/mL), 1 μL, 2 μL, 3 μL of transfection reagent (HY-K2016)
    Detection scheme: Detect the transfection effect of EGFP green fluorescent protein using a fluorescence microscope 4 h after transfection
    The results showed that different ratios of Protein Transfection Reagent (HY-K2016) showed high transfection efficiency for EGFP protein transfection.

    • Description

    • Storage

    • Components

    • Documentation

    Description
    & Advantages

    MCE Protein Transfection Reagent is a cationic lipid mixture for complexation with proteins, peptides, antibodies and other biologically active molecules to allow their direct intracellular delivery. Reagent protein complexes attach to negatively charged cell surfaces and either directly fuses with the membrane to deliver the captured protein into the cell or be endocytosed by the cell and then fuse with the endosome, releasing the captured protein into the cytoplasm.

     

    Features of MCE Protein Transfection Reagent

    1. Fast protein delivery: Optimum delivery in 3 to 12 h after incubation, with high transfection efficiency for primary cells and difficult-to-transfect cells.

    2. High protein activity: Delivery complex is non-covalent, allowing proteins and peptides retain biological activity after delivery.

    3. Versatility: Suitable for a wide range of cells, works with proteins, peptides and fluorescently labeled antibodies.

    4. Low cytotoxic: It exhibits mild action and is biocompatible

    5. Time-saving: Eliminates the need to isolate, clone and transfect gene sequences.
    Storage

    -20℃, 1 year

    Avoid repeat freeze-thaw cycles
    Components
    Components HY-K2016-0.3 mL
    Protein Transfection Reagent 0.3 mL
    Documentation

    Manual (1010 KB) SDS (341 KB)
    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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    Product Name:
    Protein Transfection Reagent
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    HY-K2016
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