2. MCE Kits
  3. Molecular Biology
  4. Restriction Endonuclease
  5. Protein Deglycosylation Kit (for N-linked & Complex O-linked Glycans)

Protein Deglycosylation Kit (for N-linked & Complex O-linked Glycans) 

Cat. No.: HY-KE8014
Manual COA SDS Technical Support

Protein Deglycosylation Kit (for N-linked & Complex O-linked Glycans) includes the enzymes and reagents reuired for the removal of all N-linked and some complex O-linked glycans. The enzyme reagent in this kit is a mixture of five glycosidases, namely PNGase F, O-Glycosidase, α2-3,6,8,9 Neuraminidase, β1-4 Galactosidase, and β-N-Acetylhexosaminidase. All enzymes in this kit are recombinant enzymes expressed in Escherichia coli BL21 and subsequently purified. The molecular weights of these enzymes are as follows: PNGase F is 36 kDa, O-Glycosidase is 14 kDa,α2-3,6,8,9 Neuraminidase is 66 kDa, β1-4 Galactosidase is 94 kDa, and β-N-Acetylhexosaminidase is 55 kDa.

Protein Deglycosylation Kit (for N-linked & Complex O-linked Glycans)
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  • Storage

  • Components

  • Documentation

Storage

Do not freeze at 4°C, 2 years.
Components
Components HY-KE8014-1 kit
PNGase F (Glycerol-free) 15000 U ( 30 μL )
O-Glycosidase 1200000 U ( 30 μL )
α2-3,6,8,9 Neuraminidase 0.6 U ( 30 μL )
β1-4 Galactosidase 0.06 U ( 30 μL )
β-N-acetylhexosaminidase 1.5 U ( 30 μL )
10×Denaturing buffer /
10×Glyco buffer /
10% NP-40 /
Documentation

Manual (2207 KB) SDS (4624 KB)
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Protein Deglycosylation Kit (for N-linked & Complex O-linked Glycans)
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