2. MCE Kits
  3. Cell Biology
  4. Cell Culture
  5. Cell Culture Supplements
  6. N-2 Supplement (100×), Sterile

N-2 Supplement (100×), Sterile 

Cat. No.: HY-K3012
Manual COA SDS Technical Support

N-2 Supplement promotes cell growth and survival. It has been associated with the growth and expression of neuroblastoma, and with post-mitotic neuronal survival and expression in primary cultures of both the peripheral nervous system (PNS) and central nervous system (CNS).

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1 Publications Citing Use of MCE N-2 Supplement (100×), Sterile

    MedChemExpress Validation
    Type: Human ovarian cancer organoids
    Matrigel : Cell ratio = 1:1
    Inoculation amount: 1500 cells/well
    Culture conditions: 37 ℃, CO2 incubator
    The results showed that N-2 Supplement (100×), Sterile (HY-K3012) has a good promoting effect on the proliferation of human ovarian cancer organoids.

    • Description

    • Storage

    • Components

    • Documentation

    Description
    & Advantages

    N-2 Supplement promotes cell growth and survival. It has been associated with the growth and expression of neuroblastoma, and with post-mitotic neuronal survival and expression in primary cultures of both the peripheral nervous system (PNS) and central nervous system (CNS). It is primarily used in the cultivation of neural cell lines and in the culture and directed differentiation of pluripotent stem cells. It can be used in conjunction with growth factors such as bFGF and EGF in serum-free neuronal cell culture mediums, DMEM and the DMEM/F12.

     

    MCE N-2 Supplement (100×), Sterile is a chemically-defined, serum-free supplement based on Bottenstein’s N-1 formulation, the solution contains transferrin, recombinant insulin, luteinizing hormone, putrescine, and selenite.
    Storage

    -20℃，2 years

    Keep away from light
    Components
    Components HY-K3012-5 mL HY-K3012-10 mL HY-K3012-50 mL
    N-2 Supplement (100×), Sterile 5 mL 2 × 5 mL 10 × 5 mL
    Documentation

    Manual (2522 KB) SDS (10313 KB)
    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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