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  5. Cell Culture Supplements
  6. N-2 Serum-free Supplement (100×)

N-2 Serum-free Supplement (100×) 

Cat. No.: HY-K3012A
Manual COA SDS Technical Support

MCE N-2 Serum-free Supplement (100×) can be used in conjunction with growth factors such as bFGF and EGF in serum-free neuronal cell culture mediums, DMEM and the DMEM/F12.

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    MedChemExpress Validation
    Cell line: B104 cells (rat neuroblastoma cells)
    Culture conditions: 37℃, 5% CO2 incubator
    Initial inoculation cell volume: 2 × 104/well (48-well plate)
    Culture medium: DMEM + 0.5% FBS + N2 (50×) + 1% PS
    Culture time: 5-6 Days
    The results showed that N-2 Serum-free Supplement (100×) (HY-K3012A ) can significantly promote the growth of B104 cells, with no significant difference compared with Competitor G.

    MedChemExpress Validation
    Cell line: SD rat cortical astrocyte primary cells
    Culture conditions: 37℃, 5% CO2 incubator
    Initial inoculation cell amount: 3 × 104/well (48-well plate)
    Culture medium: DMEM + 0.5% FBS + N2 (50×) + 1% PS + EGF (2 ng/mL)
    Culture time: 7 Days
    The results showed that N-2 Serum-free Supplement (100×) (HY-K3012A) can significantly promote the growth of SD rat cortical astrocyte primary cells, and has certain advantages over Competitor G.

    • Description

    • Storage

    • Components

    • Documentation

    Description
    & Advantages

    N-2 Supplement promotes cell growth and survival. It has been associated with the growth and expression of neuroblastoma, and with post-mitotic neuronal survival and expression in primary cultures of both the peripheral nervous system (PNS) and central nervous system (CNS). It is primarily used in the cultivation of neural cell lines and in the culture and directed differentiation of pluripotent stem cells. It can be used in conjunction with growth factors such as bFGF and EGF in serum-free neuronal cell culture mediums, DMEM and the DMEM/F12.

    MCE N-2 Serum-free Supplement (100×) is an upgraded version of Bottenstein’s N-1 formulation and is a serum-free additive with clearly defined chemical components. It contains transferrin, recombinant insulin, progesterone, putrescine, and selenite, among others. The recommended working concentration in the cell culture medium is 1×.
    Storage

    -20℃，2 years

    Keep away from light
    Components
    Components HY-K3012A-5 mL HY-K3012A-10 mL HY-K3012A-50 mL
    N-2 Serum-free Supplement (100×) 5 mL 2 × 5 mL 10 × 5 mL
    Documentation

    Manual (1046 KB) SDS (4570 KB)
    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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    N-2 Serum-free Supplement (100×)
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