2. MCE Kits
  3. Cell Biology
  4. 3D Cell Culture
  5. Basement Membrane Matrix
  6. Basement Membrane Matrix GFR

Basement Membrane Matrix GFR 

Cat. No.: HY-K6004
Manual COA SDS Technical Support

MCE Basement Membrane Matrix GFR (Growth Factor Reduced) is primarily composed of a natural basement membrane matrix derived from mouse tumors and contains less various cytokines than the standard type.This product is mainly used for organoid culture. It is also for research applications with high requirements for basement membrane preparation and has no effect on subsequent experiments requiring color detection.

Size Price Stock Quantity
Free Sample (1 mL)   Apply Now  
5 mL In-stock
10 mL In-stock
or Bulk Inquiry

* Please select Quantity before adding items.

1 Publications Citing Use of MCE Basement Membrane Matrix GFR

    MedChemExpress Validation
    Basement Membrane Matrix GFR (HY-K6004) is used for culturing mouse small intestine organoids, which enables stable organoid culture with minimal batch-to-batch variation and consistent passaging.

    • Description

    • Storage

    • Protocol

    • Components

    • Documentation

    Description
    & Advantages

    MCE Basement Membrane Matrix GFR (Growth Factor Reduced) is a natural basement membrane matrix extracted from mouse tumors and is composed mainly of various growth factors and extracellular matrix components. The main extracellular matrix components are: Laminin, Col-IV, Entactin, Heparan sulphate proteoglycans, etc. The main growth factor components are: insulin-like growth factor (IGF-1), transforming growth factor beta (TGF-β), vascular endothelial growth factor (VEGF), epidermal growth factor (EGF), fibroblast growth factor (bFGF), etc. The percentage of each of these growth factors is lower than that of standard basement membrane matrix.

    MCE Basement Membrane Matrix GFR (Growth Factor Reduced) reduces the background signal induced by growth factors and is suitable for organoid culture. It is also for research applications with high requirements for basement membrane preparation and has no effect on subsequent experiments requiring color detection.
    Storage

    -20°C, 2 years.

    Avoid repeated freezing and thawing.
    Protocol

    a. Use a pre-cooled pipette to blow the matrix gel evenly after thawing.

    b. Use pre-cooled serum-free medium to dilute according to experimental requirements. Refer to the Experimental Conditions table for final concentrations.

    c. Place the culture plate on ice and inject the substrate gel dilution slowly against the wall to avoid air bubbles. Refer to the Experimental Conditions table for the volume injected.

    d. Transfer the culture plate to 37℃ incubator gently. After gelling, aspirate off excess supernatant. Refer to the Experimental Conditions table for gel time.

     

    Note: When thawing the product for the first time, immerse the vial in crushed ice and place it in a 4°C to melt. After melting, mix thoroughly before aliquoting, and then store at -20°C.
    Components
    Components HY-K6004-5 mL HY-K6004-10 mL
    Basement Membrane Matrix GFR 5 mL 10 mL
    Documentation

    Manual (222 KB) SDS (105 KB)
    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Your Recently Viewed Products:

    Bulk Inquiry

    Your information is safe with us. * Required Fields.

    Product Name

    		  

    Requested Quantity *

    Applicant Name *

    		 

    Salutation

    Email Address *

    Phone Number *

    Department

    		 

    Organization Name *

    City

    State

    Country or Region *

    		      

    Remarks

    Bulk Inquiry

    Inquiry Information

    Product Name:
    Basement Membrane Matrix GFR
    Cat. No.:
    HY-K6004
    Quantity:
    MCE Japan Authorized Agent:

    Request for COA Report

    Please fill out this form to request the COA report. We will send it to your email within 3 working days. Your information is safe with us. * Required Fields.

    .

    Product Name

    		  

    Lot # *

    Applicant Name *

    		 

    Email Address *

    Phone Number

    		 

    Department *

    Organization Name *

    		 

    Remarks

    Request for COA Report

    We have received your request and will respond to you as soon as possible.