Exosome-Specific 293 Cell Medium Supplement 

MCE Exosome-Specific 293 Cell Medium Supplement is designed as a feed supplement for use with the Exosome-Specific 293 Cell Medium (Serum-Free) (MCE Cat. No.: HY-K3113). It is suitable for the development and production of exosomes derived from HEK293 cells. When used in combination with the Exosome-Specific 293 Cell Medium (Serum-Free), this supplement eliminates the need for serum during cell culture and significantly enhances exosome secretion by 293 cells. Compared to conventional HEK293 culture media, exosome yield can be increased by 5 to 10-fold.

4°C, 6 months.

Protect from light

1. Expand the HEK293 suspension cells to a density of 6 × 10⁶ - 8 × 10⁶ cells/mL.

2. Add the supplement at 25% of the culture volume. Continue culturing for 6-16 h, and collect the culture supernatant before cell viability drops below 90%.

3. The collected culture supernatant can be used for exosome isolation and purification.

1. This product is a sterile solution, pay attention to aseptic operation to avoid contamination.

2. This product is free of animal-derived components, growth factors, and proteins.

3. This product is for R&D use only, not for drug, household, or other uses.

4. For your safety and health, please wear a lab coat and disposable gloves to operate.