2. Metabolic Enzyme/Protease Anti-infection Apoptosis
  3. Adenosine Deaminase Bacterial Caspase
  4. Hibifolin

Hibifolin is a flavonol glycoside that can be isolated from Helicteres isora. Hibifolin is an inhibitor of adenosine deaminase (ADA) (Ki = 49.92 μM). Hibifolin protects neurons against β-amyloid-induced neurotoxicity. Hibifolin possesses a potent protective activity against cell death induced by aggregated Aβ. Hibifolin can abolish Aβ-induced caspase-3 and caspase-7 activation. Hibifolin induces Akt phosphorylation in cortical neurons. Hibifolin is also a natural sortase A (SrtA) inhibitor (IC50 = 31.2 μM) through direct binding to SrtA protein. Hibifolin attenuates the pathogenic behavior of Staphylococcus aureus including adhesion, invasion, and biofilm formation. Hibifolin improves the survival of pneumonia induced by Staphylococcus aureus in mouse model and alleviates pathological damage. Hibifolin shows a synergistic antibacterial effect with Cefotaxime (HY-A0088A).

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Hibifolin Chemical Structure

Hibifolin Chemical Structure

CAS No. : 55366-56-8

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
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Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of Hibifolin:

Hibifolin Related Antibodies

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  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Hibifolin is a flavonol glycoside that can be isolated from Helicteres isora. Hibifolin is an inhibitor of adenosine deaminase (ADA) (Ki = 49.92 μM). Hibifolin protects neurons against β-amyloid-induced neurotoxicity. Hibifolin possesses a potent protective activity against cell death induced by aggregated Aβ. Hibifolin can abolish Aβ-induced caspase-3 and caspase-7 activation. Hibifolin induces Akt phosphorylation in cortical neurons. Hibifolin is also a natural sortase A (SrtA) inhibitor (IC50 = 31.2 μM) through direct binding to SrtA protein. Hibifolin attenuates the pathogenic behavior of Staphylococcus aureus including adhesion, invasion, and biofilm formation. Hibifolin improves the survival of pneumonia induced by Staphylococcus aureus in mouse model and alleviates pathological damage. Hibifolin shows a synergistic antibacterial effect with Cefotaxime (HY-A0088A)[1][2][3].

IC50 & Target

Ki: 49.92 μM (ADA)[1]
In Vitro

Hibifolin (100 μM) shows about 60% inhibition of the ADA (10 μM) enzymatic activity[1].
Hibifolin (0.5-50 μM, 24 h) reduces the release of LDH induced by Aβ in cortical neurons[2].
Hibifolin (50 μM, 0-120 sec) reduces the amount of Aβ-induced Ca2+ by 70% in cortical neurons[2].
Hibifolin (50 μM) induces the phosphorylation of Akt to approximately 2-fold[2].
Hibifolin (0-1000 μg/mL) remarkably inhibits the activity of SrtA with an IC50 of 31.2 μM[3].
Hibifolin (32-256 μg/mL) decreases the adhesion of S. fibrinogen to fribinogen with the concentration of 256 μg/mL and significantly inhibits bacterial invasion of A549 cells[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Hibifolin Related Antibodies

Cell Viability Assay[2]

Cell Line: Cortical neurons
Concentration: 0.5, 5, 50 μM
Incubation Time: 24 h
Result: Had protective effect by preventing Aβ-induced cell death dose-dependently.

Western Blot Analysis[2]

Cell Line: Cortical neurons
Concentration: 50 μM
Incubation Time: 24 h
Result: Blocked the DNA fragmentation triggered by Aβ.
Molecular Weight

494.36

Formula

C21H18O14
CAS No.
Appearance

Solid

Color

Yellow to orange

SMILES

O[C@H]([C@H]([C@@H]([C@@H](C(O)=O)O1)O)O)[C@@H]1OC2=C3C(C(C(O)=C(C4=CC=C(O)C(O)=C4)O3)=O)=C(O)C=C2O
Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (202.28 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.0228 mL 10.1141 mL 20.2282 mL
5 mM 0.4046 mL 2.0228 mL 4.0456 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
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Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

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C2

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Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.5 mg/mL (5.06 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: 99.70%

SDS (393 KB)

COA (253 KB) HNMR (134 KB) CNMR (122 KB) RP-HPLC (181 KB) MS (428 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.0228 mL 10.1141 mL 20.2282 mL 50.5704 mL
5 mM 0.4046 mL 2.0228 mL 4.0456 mL 10.1141 mL
10 mM 0.2023 mL 1.0114 mL 2.0228 mL 5.0570 mL
15 mM 0.1349 mL 0.6743 mL 1.3485 mL 3.3714 mL
20 mM 0.1011 mL 0.5057 mL 1.0114 mL 2.5285 mL
25 mM 0.0809 mL 0.4046 mL 0.8091 mL 2.0228 mL
30 mM 0.0674 mL 0.3371 mL 0.6743 mL 1.6857 mL
40 mM 0.0506 mL 0.2529 mL 0.5057 mL 1.2643 mL
50 mM 0.0405 mL 0.2023 mL 0.4046 mL 1.0114 mL
60 mM 0.0337 mL 0.1686 mL 0.3371 mL 0.8428 mL
80 mM 0.0253 mL 0.1264 mL 0.2529 mL 0.6321 mL
100 mM 0.0202 mL 0.1011 mL 0.2023 mL 0.5057 mL
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Hibifolin Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Hibifolin55366-56-8Adenosine DeaminaseBacterialCaspaseadenosinedeaminaseanti-inflammatoryanti-proliferativeneurotoxicityInhibitorinhibitorinhibit

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