2. PI3K/Akt/mTOR Immunology/Inflammation Anti-infection
  3. PI3K Akt Interleukin Related Bacterial
  4. FHND5032

FHND5032 is an orally active miR-124 inducer. FHND5032 significantly upregulates miR-124 expression in macrophages. FHND5032 disrupts inflammatory signaling, promotes macrophage reprogramming, and restores the epithelial barrier function by inhibiting the PIK3R2/PI3K/Akt axis. FHND5032 alleviates colitis and reduces inflammatory burden in ulcerative colitis mice. FHND5032 can be used for the study of ulcerative colitis.

For research use only. We do not sell to patients.

FHND5032 Chemical Structure

FHND5032 Chemical Structure

CAS No. : 3076057-63-8

Size Stock
50 mg   Get quote  
100 mg   Get quote  
250 mg   Get quote  

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

FHND5032 Related Antibodies

Top Publications Citing Use of Products

View All PI3K Isoform Specific Products:

View All Isoforms
PI3Kα PI3Kβ PI3Kγ PI3Kδ PI3KC2α PI3KC2β PI3KC2γ Vps34 PI3K PI3KC3 p120γ

View All Akt Isoform Specific Products:

View All Isoforms
Akt Akt1 Akt2 Akt3

View All Interleukin Related Isoform Specific Products:

View All Isoforms
IL-1 IL-2 IL-3 IL-4 IL-5 IL-6 IL-8 IL-10 IL-12 IL-13 IL-15 IL-17 IL-23 IL-7 IL-33 IL-22

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

FHND5032 is an orally active miR-124 inducer. FHND5032 significantly upregulates miR-124 expression in macrophages. FHND5032 disrupts inflammatory signaling, promotes macrophage reprogramming, and restores the epithelial barrier function by inhibiting the PIK3R2/PI3K/Akt axis. FHND5032 alleviates colitis and reduces inflammatory burden in ulcerative colitis mice. FHND5032 can be used for the study of ulcerative colitis[1].

In Vitro

FHND5032 (0-20 μM, 24 h) significantly exerts anti-inflammatory activity in THP-1 and RAW 264.7 cells by enhancing miR-124 expression[1].
FHND5032 (≤ 5 μM) activates miR-124-5p expression to regulate the PI3K/Akt pathway by targeting PIK3R2 in THP-1 and RAW 264.7 cells reprogramming[1].
FHND5032 decreases the expression of M1 marker CD86 while increasing the expression of M2 marker CD206 in RAW 264.7 cells[1].
FHND5032 promots the expression of the barrier-sealing proteins ZO-1 and occludin to facilitate Tight junction (TJ) assembly in Caco-2 cells[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

FHND5032 Related Antibodies

Real Time qPCR[1]

Cell Line: THP-1 and RAW 264.7 cells
Concentration: 5, 10 μM
Incubation Time: /
Result: Upregulated miR-124 levels and induced miR-124 expression, which peaked at a concentration of 5 μM.
Decreased TNF-α, IL-1β, and IL-6 expression in LPS (HY-D1056)- and IFN-γ-stimulated THP-1 cells and LPS stimulated RAW 264.7 cells.
Decreased the expression of PIK3R2
In Vivo

FHND5032 (25-50 mg/kg, i.g., daily, 12 days) alleviates colitis and reduces inflammatory burden in Dextran sulfate sodium salt (DSS) (HY-116282C)-induced ulcerative colitis (UC) mice[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: DSS-induced female C57BL/6J mice (18-20 g)[1]
Dosage: 25, 50 mg/kg
Administration: i.g. daily for 12 days
Result: Restrained the body weight loss of DSS-induced colitis mice.
Reduced the hematochezia occurrence during treatment.
Showed longer colon length of mice.
Protected the colonic barrier from pathological damage.
Decreased the local level of UC-associated myeloperoxidase (MPO) in the colon.
Reduced the expression of pro-inflammatory cytokines, including IL-6, TNF-α, and IFN-γ.
Increased the expression of anti-inflammatory cytokines IL-10.
Prevented intestinal damage in UC through elevation of TJ proteins.
Prevented the FITC-dextran leakage.
Inhibited colonic inflammation and repaired the intestinal barrier in colitis mice.
Upregulated the miR-124 expression and decreased PIK3R2 expression.
Attenuated PIK3R2 upregulation and the hyperphosphorylation of PI3K and Akt.
Showed no significant discrepancies in serum physicochemical parameters (alanine aminotransferase (ALT), aspartate aminotransferase (AST), blood urea nitrogen (BUN), and creatinine (Cr)).
Decreased the abundance of Proteobacteria in colitis mice and effectively enhanced the relative abundance of Firmicutes.
Showed no increase on the abundance of Bacteroidota.
Molecular Weight

339.70

Formula

C15H9ClF3N3O
CAS No.
SMILES

FC(F)(OC1=CC=C(C=C1)NC2=NC3=CC(Cl)=CC=C3C=N2)F
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
  • No file chosen (Maximum size is: 1024 Kb)
  • If you have published this work, please enter the PubMed ID.
  • Your name will appear on the site.

FHND5032 Related Classifications

  • Molarity Calculator

  • Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass   Concentration   Volume   Molecular Weight *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

FHND50323076057-63-8FHND 5032FHND-5032PI3KAktInterleukin RelatedBacterialPhosphoinositide 3-kinasePKBProtein kinase BILmiR-124ulcerative colitis,THP-1 cellsRAW 264.7 cellsInhibitorinhibitorinhibit

Your Recently Viewed Products:

Inquiry Online

Your information is safe with us. * Required Fields.

Product Name

  

Requested Quantity *

Applicant Name *

 

Salutation

Email Address *

 

Phone Number *

Department

 

Organization Name *

City

State

Country or Region *

      

Remarks

Bulk Inquiry

Inquiry Information

Product Name:
FHND5032
Cat. No.:
HY-174976
Quantity:
MCE Japan Authorized Agent:

Customer Review

Thank You for Your Feedback!

Request for HNMR Report

Please fill out this form to request the QC report. We will send it to your Email address shortly.

Your information is safe with us. * Required Fields.

Product Name

  

Lot #

Applicant Name *

 

Email Address *

Phone Number

 

Department *

Organization Name *

 

Country or Region *

Remarks

SAFETY DATA SHEET (SDS)

Request for HNMR Report

We have received your request and will respond to you as soon as possible.