2. Immunology/Inflammation GPCR/G Protein Neuronal Signaling MAPK/ERK Pathway Stem Cell/Wnt
  3. COX Melanocortin Receptor ERK
  4. Fenoprofen

Fenoprofen (LILLY-53858) is a nonsteroidal anti-inflammatory agent (NSAID) and inhibits cyclooxygenase (COX). Fenoprofen is a melanocortin receptors (MCRs) positive allosteric modulator (PAM). Fenoprofen also increases ERK1/2 activation in HEK293T cells. Fenoprofen has anti-arthritic activities and can be used for the study of rheumatoid arthritis and osteoarthritis.

For research use only. We do not sell to patients.

Fenoprofen Chemical Structure

Fenoprofen Chemical Structure

CAS No. : 29679-58-1

Size Price Stock Quantity
Solid or liquid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
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Solution
10 mM * 1 mL in DMSO In-stock
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Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of Fenoprofen:

Fenoprofen Related Antibodies

Top Publications Citing Use of Products

1 Publications Citing Use of MCE Fenoprofen

View All COX Isoform Specific Products:

View All Isoforms
COX COX-1 COX-2 COX-3

View All Melanocortin Receptor Isoform Specific Products:

View All Isoforms
MC1R MC3R MC2R MC4R MC5R

View All ERK Isoform Specific Products:

View All Isoforms
ERK ERK1 ERK2 ERK5 ERK8

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Fenoprofen (LILLY-53858) is a nonsteroidal anti-inflammatory agent (NSAID) and inhibits cyclooxygenase (COX). Fenoprofen is a melanocortin receptors (MCRs) positive allosteric modulator (PAM). Fenoprofen also increases ERK1/2 activation in HEK293T cells. Fenoprofen has anti-arthritic activities and can be used for the study of rheumatoid arthritis and osteoarthritis[1][2].

In Vitro

In HEK293T cells, Fenoprofen stimulates ERK1/2 activation at MC3-5R in a biphasic manner, with the highest ERK1/2 activation observed at concentrations of 3 and 10 µM for MC3R, 1 µM for MC4R, and 3 µM for MC5R. No significant difference of Fenoprofen-induced pERK1/2 levels was observed in cells transfected with the empty vector pcDNA3.1 as well as MC1R. Fenoprofen induces biased signaling at MC3-5R, as it selectively activated ERK1/2 cascade but not the canonical cAMP signaling[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Fenoprofen Related Antibodies
In Vivo

Fenoprofen (10 mg/kg; i.p.; twice per day from day 2; for 7 days) presents anti-arthritic actions on cartilage integrity and synovitis, effects markedly attenuated in Mc3r-/- mice[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Male C57BL/6J wild-type (WT) mice (7-8 weeks old) and Mc3r-/- mice injected with the K/BxN serum[2]
Dosage: 10 mg/kg
Administration: i.p.; twice per day from day 2; for 7 days
Result: Reduced paw volume and reduced disease incidence.
Molecular Weight

242.27

Formula

C15H14O3
CAS No.
Appearance

Viscous Liquid

Color

Colorless to light yellow

SMILES

O=C(O)C(C)C1=CC=CC(OC2=CC=CC=C2)=C1
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Pure form -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (412.76 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 4.1276 mL 20.6378 mL 41.2756 mL
5 mM 0.8255 mL 4.1276 mL 8.2551 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (10.32 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.5 mg/mL (10.32 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: 99.70%

SDS (393 KB)

COA (245 KB) HNMR (278 KB) LCMS (98 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 4.1276 mL 20.6378 mL 41.2756 mL 103.1890 mL
5 mM 0.8255 mL 4.1276 mL 8.2551 mL 20.6378 mL
10 mM 0.4128 mL 2.0638 mL 4.1276 mL 10.3189 mL
15 mM 0.2752 mL 1.3759 mL 2.7517 mL 6.8793 mL
20 mM 0.2064 mL 1.0319 mL 2.0638 mL 5.1594 mL
25 mM 0.1651 mL 0.8255 mL 1.6510 mL 4.1276 mL
30 mM 0.1376 mL 0.6879 mL 1.3759 mL 3.4396 mL
40 mM 0.1032 mL 0.5159 mL 1.0319 mL 2.5797 mL
50 mM 0.0826 mL 0.4128 mL 0.8255 mL 2.0638 mL
60 mM 0.0688 mL 0.3440 mL 0.6879 mL 1.7198 mL
80 mM 0.0516 mL 0.2580 mL 0.5159 mL 1.2899 mL
100 mM 0.0413 mL 0.2064 mL 0.4128 mL 1.0319 mL
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Fenoprofen Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Fenoprofen29679-58-1LILLY-53858LILLY53858LILLY 53858COXMelanocortin ReceptorERKCyclooxygenaseMC ReceptorExtracellular signal regulated kinasesanti-inflammatory agentNSAIDarthritisosteoarthritisrheumatoid arthritisinflammationswellingstiffnessjoint painallosteric enhancermelanocortin receptorincreases ERK1/2 activationInhibitorinhibitorinhibit

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