2. Apoptosis NF-κB Metabolic Enzyme/Protease Immunology/Inflammation Neuronal Signaling
  3. Apoptosis Reactive Oxygen Species (ROS) Caspase Cholinesterase (ChE)
  4. Fenobucarb

Fenobucarb is a carbamate insecticide. Fenobucarb induces zebrafish developmental neurotoxicity through pathways involved in inflammation, oxidative stress, degeneration and apoptosis. Fenobucarb is a possible risk factor to cardiovascular and cerebrovascular systems in animals.

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Fenobucarb

Fenobucarb Chemical Structure

CAS No. : 3766-81-2

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Oil + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
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Based on 1 publication(s) in Google Scholar

Other Forms of Fenobucarb:

Fenobucarb Related Antibodies

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  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Fenobucarb is a carbamate insecticide. Fenobucarb induces zebrafish developmental neurotoxicity through pathways involved in inflammation, oxidative stress, degeneration and apoptosis. Fenobucarb is a possible risk factor to cardiovascular and cerebrovascular systems in animals[1][2].

IC50 & Target[1]

Caspase 3

 

Caspase-7

 

Caspase-9

 

AChE

 

In Vitro

Fenobucarb (6.25-200 μg/mL, 6-120 hours post fertilization (hpf)) dose dependently induces apoptosis, significantly increases Caspase activity and reactive oxygen species levels, and significantly inhibits AChE activity in wild-type AB zebrafish brain cells[1].
Fenobucarb (12.5-200 μg/mL, 120 hbf) significantly downregulate multiple neurodevelopmental genes in zebrafish[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Fenobucarb Related Antibodies

RT-PCR[1]

Cell Line: Zebrafish
Concentration: 12.5, 25, 50, 100 and 200 μg/mL
Incubation Time: 120 hbf
Result: Significantly reduced the mRNA levels of mbp, gfap and shha.
In Vivo

Fenobucarb (6.25–400 μg/mL, 6-120 hbf) causes degeneration of central neurons, shortening of motor neuron length, increased infiltration of neutrophils, significant decrease in movement distance and speed in zebrafish (LC50 = 205 μg/mL)[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Molecular Weight

207.27

Formula

C12H17NO2
CAS No.
Appearance

Oil

Color

Colorless to light yellow

SMILES

CCC(C1=CC=CC=C1OC(NC)=O)C
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Pure form -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (482.46 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 4.8246 mL 24.1228 mL 48.2456 mL
5 mM 0.9649 mL 4.8246 mL 9.6491 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (12.06 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.5 mg/mL (12.06 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 4.8246 mL 24.1228 mL 48.2456 mL 120.6139 mL
5 mM 0.9649 mL 4.8246 mL 9.6491 mL 24.1228 mL
10 mM 0.4825 mL 2.4123 mL 4.8246 mL 12.0614 mL
15 mM 0.3216 mL 1.6082 mL 3.2164 mL 8.0409 mL
20 mM 0.2412 mL 1.2061 mL 2.4123 mL 6.0307 mL
25 mM 0.1930 mL 0.9649 mL 1.9298 mL 4.8246 mL
30 mM 0.1608 mL 0.8041 mL 1.6082 mL 4.0205 mL
40 mM 0.1206 mL 0.6031 mL 1.2061 mL 3.0153 mL
50 mM 0.0965 mL 0.4825 mL 0.9649 mL 2.4123 mL
60 mM 0.0804 mL 0.4020 mL 0.8041 mL 2.0102 mL
80 mM 0.0603 mL 0.3015 mL 0.6031 mL 1.5077 mL
100 mM 0.0482 mL 0.2412 mL 0.4825 mL 1.2061 mL
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Fenobucarb Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Fenobucarb3766-81-2ApoptosisReactive Oxygen Species (ROS)CaspaseCholinesterase (ChE)insecticidezebrafishapoptosisneurotoxicitycerebrovascularROScaspaseInhibitorinhibitorinhibit

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