2. Neuronal Signaling Membrane Transporter/Ion Channel GPCR/G Protein
  3. Cholinesterase (ChE) iGluR mAChR
  4. Ethopropazine hydrochloride

Ethopropazine hydrochloride  (Synonyms: Isothazine)

Cat. No.: HY-107922 Purity: 99.66%
COA
SDS
Handling Instructions Technical Support

Ethopropazine (Isothazine) hydrochloride is a potent and selective BChE inhibitor, a poor AChE inhibitor and a non-selective mAChR and NMDA antagonist. Ethopropazine hydrochloride has anticholinergic, antihistamine, antiadrenergic actions and properties. Ethopropazine hydrochloride alleviates thermal hyperalgesia in a dose dependent manner. Ethopropazine hydrochloride can be used for the research of Parkinson’s disease.

For research use only. We do not sell to patients.

Ethopropazine hydrochloride

Ethopropazine hydrochloride Chemical Structure

CAS No. : 1094-08-2

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
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Solution
10 mM * 1 mL in DMSO In-stock
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Based on 1 publication(s) in Google Scholar

Other Forms of Ethopropazine hydrochloride:

Ethopropazine hydrochloride Related Antibodies

Top Publications Citing Use of Products

1 Publications Citing Use of MCE Ethopropazine hydrochloride

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mAChR1 mAChR2 mAChR3 mAChR4 mAChR5

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Ethopropazine (Isothazine) hydrochloride is a potent and selective BChE inhibitor, a poor AChE inhibitor and a non-selective mAChR and NMDA antagonist. Ethopropazine hydrochloride has anticholinergic, antihistamine, antiadrenergic actions and properties. Ethopropazine hydrochloride alleviates thermal hyperalgesia in a dose dependent manner. Ethopropazine hydrochloride can be used for the research of Parkinson’s disease[1][2][3][4][5].

IC50 & Target

AChE

 

BChE

 

In Vitro

Ethopropazine (0-250 µM, 24 h) hydrochloride exhibits dose-dependent toxicity in LAN-5 cells with an LD50 of 28 µM[1].
Ethopropazine (<28 µM, 24 h) hydrochloride exhibits proliferative properties in SK-N-SH cells by enhancing [3H] thymidine uptake[1].
Ethopropazine (10 μM, 24 h) hydrochloride significantly enhances the proliferation of NCI-H69 cells, and this proliferative effect is significantly inhibited by the mAChR3 antagonist 4-DAMP (HY-100958)[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Ethopropazine hydrochloride Related Antibodies
In Vivo

Ethopropazine (10-30 mg/kg, subcutaneous administration, single dose) hydrochloride can alleviate neuropathic pain in rats in a dose-dependent manner, and combination with α2 agonists can enhance analgesic efficacy while maintaining safety[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Adult female Sprague-Dawley rats (weight 300-330 g) underwent loose ligation of the right sciatic nerve to induce thermal hyperalgesia (neuropathic pain)[1]
Dosage: 10, 20, 30 mg/kg combined with 0.025 mg/kg Clonidine (HY-12721) or 0.1 mg/kg Guanabenz (HY-12724)
Administration: Subcutaneous administration for a single dose
Result: Showed no significant effect on pawwithdrawal latency (PWL) in either ligated or unligated limbs at 10 mg/kg.
Increased PWL in the ligated limb significantly at 1 hour post-administration at 20 mg/kg.
Increased PWL in the ligated limb at 1, 2, and 3 hours post-administration at 30 mg/kg.
Increased PWL in the ligated limb at 1-2 hours post-administration when combined with 0.025 mg/kg Clonidine.
Increased PWL in the ligated limb at 1-3 hours post-administration when combined with 0.1 mg/kg Guanabenz.
Molecular Weight

348.93

Formula

C19H25ClN2S
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

CCN(CC)C(C)CN1C2=CC=CC=C2SC3=CC=CC=C13.[H]Cl
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility
In Vitro: 

DMSO : 25 mg/mL (71.65 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.8659 mL 14.3295 mL 28.6590 mL
5 mM 0.5732 mL 2.8659 mL 5.7318 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (7.16 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.5 mg/mL (7.16 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: 99.66%

SDS (392 KB)

COA (253 KB) HNMR (255 KB) RP-HPLC (262 KB) MS (68 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.8659 mL 14.3295 mL 28.6590 mL 71.6476 mL
5 mM 0.5732 mL 2.8659 mL 5.7318 mL 14.3295 mL
10 mM 0.2866 mL 1.4330 mL 2.8659 mL 7.1648 mL
15 mM 0.1911 mL 0.9553 mL 1.9106 mL 4.7765 mL
20 mM 0.1433 mL 0.7165 mL 1.4330 mL 3.5824 mL
25 mM 0.1146 mL 0.5732 mL 1.1464 mL 2.8659 mL
30 mM 0.0955 mL 0.4777 mL 0.9553 mL 2.3883 mL
40 mM 0.0716 mL 0.3582 mL 0.7165 mL 1.7912 mL
50 mM 0.0573 mL 0.2866 mL 0.5732 mL 1.4330 mL
60 mM 0.0478 mL 0.2388 mL 0.4777 mL 1.1941 mL
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Ethopropazine hydrochloride Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Ethopropazine1094-08-2IsothazineCholinesterase (ChE)iGluRmAChRIonotropic glutamate receptorsMuscarinic acetylcholine receptorEthopropazine (Isothazine) hydrochlorideParkinson’s diseaseCholinesterasehyperalgesiaInhibitorinhibitorinhibit

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