2. PI3K/Akt/mTOR
  3. mTOR
  4. eCF309

eCF309 is a potent and highly selective mTOR inhibitor with an IC50 of 15 nM. eCF309 displays higher selectivity over PI3Ks. eCF309 can be used for the study of breast cancer and prostate cancer.

For research use only. We do not sell to patients.

eCF309 Chemical Structure

eCF309 Chemical Structure

CAS No. : 2001571-40-8

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500 μg In-stock
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Based on 1 publication(s) in Google Scholar

eCF309 Related Antibodies

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Description

eCF309 is a potent and highly selective mTOR inhibitor with an IC50 of 15 nM. eCF309 displays higher selectivity over PI3Ks. eCF309 can be used for the study of breast cancer and prostate cancer[1].

Cellular Effect
Cell Line Type Value Description References
MCF7 EC50
8 nM
Compound: 12
Cytotoxicity against human MCF7 cells after 5 days by Presto blue reagent-based fluorescence analysis
Cytotoxicity against human MCF7 cells after 5 days by Presto blue reagent-based fluorescence analysis
10.1039/C5MD00493D
MDA-MB-231 EC50
72 nM
Compound: 12
Cytotoxicity against human MDA-MB-231 cells after 5 days by Presto blue reagent-based spectrofluorometric analysis
Cytotoxicity against human MDA-MB-231 cells after 5 days by Presto blue reagent-based spectrofluorometric analysis
10.1039/C5MD00493D
PC-3 EC50
37 nM
Compound: 12
Cytotoxicity against human PC3 cells after 5 days by Presto blue reagent-based spectrofluorometric analysis
Cytotoxicity against human PC3 cells after 5 days by Presto blue reagent-based spectrofluorometric analysis
10.1039/C5MD00493D
T98 EC50
100 nM
Compound: eCF309
Antiproliferative activity against human T98 cells assessed as reduction in cell viability incubated for 5 days by Prestoblue reagent based assay
Antiproliferative activity against human T98 cells assessed as reduction in cell viability incubated for 5 days by Prestoblue reagent based assay
[PMID: 31787462]
U-87MG ATCC EC50
100 nM
Compound: eCF309
Antiproliferative activity against human U87 cells assessed as reduction in cell viability incubated for 5 days by Prestoblue reagent based assay
Antiproliferative activity against human U87 cells assessed as reduction in cell viability incubated for 5 days by Prestoblue reagent based assay
[PMID: 31787462]
In Vitro

eCF309 (0.3-1000 nM; 5 days) exhibits highly potent antiproliferative activity against MCF7, MDA-MB-231 and PC3 cells[1].
eCF309 (0.3-1000 nM; 24 h) causes G0/G1 cell cycle arrest[1].
eCF309 (3-100 nM; 1.5 h) inhibits pP70S6K, pS6 and pAKT levels in MCF7 cells[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

eCF309 Related Antibodies

Cell Viability Assay[1]

Cell Line: MCF7, MDA-MB-231 and PC3 cells
Concentration: 0.3-1000 nM
Incubation Time: 5 days
Result: Exhibits EC50 values upon MCF7, MDA-MB-231 and PC3 cells viability of 8.4 nM, 72 nM and 37 nM, respectively.

Cell Cycle Analysis[1]

Cell Line: MCF7, MDA-MB-231 and PC3 cells
Concentration: 0.3 nM, 1 nM, 3 nM, 10 nM, 30 nM, 100 nM, 300 nM, 1000 nM
Incubation Time: 24 h
Result: Caused G0/G1 cell cycle arrest.

Western Blot Analysis[1]

Cell Line: MCF7 cells
Concentration: 3 nM, 10 nM, 30 nM, 100 nM
Incubation Time: For 30 min, followed by 1 h of serum stimulation
Result: Mediated a dose-dependent reduction of the pP70S6K, pS6 and pAKT levels in MCF7 cells, achieving almost complete inhibition of the phosphorylation of these targets at 30 nM.
Molecular Weight

383.40

Formula

C18H21N7O3
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

NC1=C2C(N(CC(OCC)OCC)N=C2C3=CC=C(OC(N)=N4)C4=C3)=NC=N1
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

-20°C, protect from light, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)

Solvent & Solubility
In Vitro: 

DMSO : 1 mg/mL (2.61 mM; Need ultrasonic and warming; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.6082 mL 13.0412 mL 26.0824 mL
5 mM --- --- ---
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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In Vivo Dissolution Calculator
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Working solution concentration: mg/mL
Purity & Documentation

Purity: 98.2%

SDS (251 KB)

COA (251 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.6082 mL 13.0412 mL 26.0824 mL 65.2061 mL
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eCF309 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

eCF3092001571-40-8eCF 309eCF-309mTORMammalian target of RapamycinMCF-7HER2breast cancerInhibitorinhibitorinhibit

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