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  3. Cyanine5 NHS ester iodide

Cyanine5 NHS ester iodide is a red emitting fluorescent dye for labeling of amino-groups in peptides, proteins, and oligonucleotides.

For research use only. We do not sell to patients.

Cyanine5 NHS ester iodide

Cyanine5 NHS ester iodide Chemical Structure

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Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of Cyanine5 NHS ester iodide:

Top Publications Citing Use of Products

1 Publications Citing Use of MCE Cyanine5 NHS ester iodide

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Cyanine5 NHS ester iodide is a red emitting fluorescent dye for labeling of amino-groups in peptides, proteins, and oligonucleotides[1].

In Vitro

Cyanine-5 NHS ester amine-reactive red emitting fluorescent dye is used to react with the free amino group on the cysteine amino acid present in terminal peptides used for end-capping Poly(β-amino esters) (pBAEs) backbones[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

707.64

Formula

C36H42IN3O4

Appearance

Solid

Color

Yellow to brown

Emission (Em)

670

Excitation (Ex)

645

SMILES

CC1(C)C(/C=C/C=C/C=C2N(C)C3=C(C=CC=C3)C\2(C)C)=[N+](CCCCCC(ON4C(CCC4=O)=O)=O)C5=C1C=CC=C5.[I-]

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility
In Vitro: 

DMSO : 110 mg/mL (155.45 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.4131 mL 7.0657 mL 14.1315 mL
5 mM 0.2826 mL 1.4131 mL 2.8263 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation

Purity: 98.02%

References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.4131 mL 7.0657 mL 14.1315 mL 35.3287 mL
5 mM 0.2826 mL 1.4131 mL 2.8263 mL 7.0657 mL
10 mM 0.1413 mL 0.7066 mL 1.4131 mL 3.5329 mL
15 mM 0.0942 mL 0.4710 mL 0.9421 mL 2.3552 mL
20 mM 0.0707 mL 0.3533 mL 0.7066 mL 1.7664 mL
25 mM 0.0565 mL 0.2826 mL 0.5653 mL 1.4131 mL
30 mM 0.0471 mL 0.2355 mL 0.4710 mL 1.1776 mL
40 mM 0.0353 mL 0.1766 mL 0.3533 mL 0.8832 mL
50 mM 0.0283 mL 0.1413 mL 0.2826 mL 0.7066 mL
60 mM 0.0236 mL 0.1178 mL 0.2355 mL 0.5888 mL
80 mM 0.0177 mL 0.0883 mL 0.1766 mL 0.4416 mL
100 mM 0.0141 mL 0.0707 mL 0.1413 mL 0.3533 mL
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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Product Name:
Cyanine5 NHS ester iodide
Cat. No.:
HY-135414B
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