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  3. Cyanine5 NHS ester chloride

Cyanine5 NHS ester chloride is a red emitting fluorescent dye for labeling of amino-groups in peptides, proteins, and oligonucleotides.

For research use only. We do not sell to patients.

Cyanine5 NHS ester chloride Chemical Structure

Cyanine5 NHS ester chloride Chemical Structure

CAS No. : 1032678-42-4

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Based on 1 publication(s) in Google Scholar

Other Forms of Cyanine5 NHS ester chloride:

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Description

Cyanine5 NHS ester chloride is a red emitting fluorescent dye for labeling of amino-groups in peptides, proteins, and oligonucleotides[1].

In Vitro

Cyanine-5 NHS ester amine-reactive red emitting fluorescent dye is used to react with the free amino group on the cysteine amino acid present in terminal peptides used for end-capping Poly(β-amino esters) (pBAEs) backbones[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

616.19

Formula

C36H42ClN3O4

CAS No.
Appearance

Solid

Color

Brown to black

SMILES

CC1(C)C(/C=C/C=C/C=C2N(C)C3=C(C=CC=C3)C\2(C)C)=[N+](CCCCCC(ON4C(CCC4=O)=O)=O)C5=C1C=CC=C5.[Cl-]

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility
In Vitro: 

DMSO : 50 mg/mL (81.14 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.6229 mL 8.1144 mL 16.2288 mL
5 mM 0.3246 mL 1.6229 mL 3.2458 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
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Concentration
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Volume
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Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

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Volume (start)

V1

=
Concentration (final)

C2

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Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.6229 mL 8.1144 mL 16.2288 mL 40.5719 mL
5 mM 0.3246 mL 1.6229 mL 3.2458 mL 8.1144 mL
10 mM 0.1623 mL 0.8114 mL 1.6229 mL 4.0572 mL
15 mM 0.1082 mL 0.5410 mL 1.0819 mL 2.7048 mL
20 mM 0.0811 mL 0.4057 mL 0.8114 mL 2.0286 mL
25 mM 0.0649 mL 0.3246 mL 0.6492 mL 1.6229 mL
30 mM 0.0541 mL 0.2705 mL 0.5410 mL 1.3524 mL
40 mM 0.0406 mL 0.2029 mL 0.4057 mL 1.0143 mL
50 mM 0.0325 mL 0.1623 mL 0.3246 mL 0.8114 mL
60 mM 0.0270 mL 0.1352 mL 0.2705 mL 0.6762 mL
80 mM 0.0203 mL 0.1014 mL 0.2029 mL 0.5071 mL
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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Product Name:
Cyanine5 NHS ester chloride
Cat. No.:
HY-135414
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