2. Anti-infection NF-κB Immunology/Inflammation
  3. SARS-CoV Virus Protease NF-κB Interleukin Related
  4. Chymostatin

Chymostatin is a SARS-CoV-2 Mpro inhibitor. Chymostatin up-regulates endocan and inhibits NF-κBp65 activity. Chymostatin reduces proinflammatory cytokines (IL-1β and IL-6) and HMGB1. Chymostatin shows protective effects against Paraquat-induced acute lung injury. Chymostatin exhibits anticancer activity against lung cancer.

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Custom Peptide Synthesis

Chymostatin

Chymostatin Chemical Structure

CAS No. : 9076-44-2

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Based on 1 publication(s) in Google Scholar

Chymostatin Related Antibodies

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  • Biological Activity

  • Purity & Documentation

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Description

Chymostatin is a SARS-CoV-2 Mpro inhibitor. Chymostatin up-regulates endocan and inhibits NF-κBp65 activity. Chymostatin reduces proinflammatory cytokines (IL-1β and IL-6) and HMGB1. Chymostatin shows protective effects against Paraquat-induced acute lung injury. Chymostatin exhibits anticancer activity against lung cancer[1][2][3][4][5][6][7].

IC50 & Target[1][2][3][4][5][6][7]

NF-κB

 

IL-6

 

IL-1β

 

In Vitro

Chymostatin (20-150 μM) inhibits cellular aggregation of activated human peripheral blood lymphocytes[2].
Chymostatin (50 μM, 1 h) combined with Aliskiren (HY-12176) to alleviate endoplasmic reticulum stress induced by lipid overload in kidney tubular cells HK2[3].
Chymostatin shows dose-dependent inhibition of COVID-19 Mpro activity with IC50 of 15.81 µM[4].
Chymostatin (up to 100 µM) does not show any cytotoxicity on normal mouse lung epithelial cells, where as it inhibits proliferation of human H441 lung cancer cells (KrasG12V mutant) with IC50 of 1.2 µM[4].
Chymostatin (20 µM) decreases protein breakdown in leg muscles from normal rodents and also in denervated and dystrophic muscles[5].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chymostatin Related Antibodies
In Vivo

Chymostatin (2 mg/kg, i.p., 30 min before paraquat administration) shows protective effects against paraquat-induced acute lung injury in mice[1].
Chymostatin (2 mg/kg/h, i.v.) decreases heart rate, renal blood flow and increases IVR in 2K1C rats[6].
Chymostatin (2 mg/kg/h, i.v.) increases plasma potassium concentrations (17%) in UNX HS rats[6].
. Chymostatin (1 mg/kg, i.p.) retards the decrease in locomotor activity and increases muscle protein in dystrophic mice[7].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Acute lung injury female mice (injected with Paraquat at a dosage of 25 mg/kg)[1]
Dosage: 2 mg/kg
Administration: Intraperitoneal injection (i.p.), 30 min before paraquat administration
Result: Did not affect the lung tissue structure.
Decreased the lung W/D ratios, the production of TNF-a, IL-1β, IL-6 and HMGB1.
Decreased the increase in cathepsin G activity.
Increased endocan concentration.
Down-regulated nuclear NF-κBp65 in the lung.
Molecular Weight

607.70

CAS No.
Appearance

Solid

Color

White to off-white

Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Sealed storage, away from moisture

Powder -80°C 2 years
-20°C 1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility
In Vitro: 

DMSO : 50 mg/mL (82.28 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.6455 mL 8.2277 mL 16.4555 mL
5 mM 0.3291 mL 1.6455 mL 3.2911 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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This equation is commonly abbreviated as: C1V1 = C2V2

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation

Purity: 99.9%

SDS (251 KB)

COA (249 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.6455 mL 8.2277 mL 16.4555 mL 41.1387 mL
5 mM 0.3291 mL 1.6455 mL 3.2911 mL 8.2277 mL
10 mM 0.1646 mL 0.8228 mL 1.6455 mL 4.1139 mL
15 mM 0.1097 mL 0.5485 mL 1.0970 mL 2.7426 mL
20 mM 0.0823 mL 0.4114 mL 0.8228 mL 2.0569 mL
25 mM 0.0658 mL 0.3291 mL 0.6582 mL 1.6455 mL
30 mM 0.0549 mL 0.2743 mL 0.5485 mL 1.3713 mL
40 mM 0.0411 mL 0.2057 mL 0.4114 mL 1.0285 mL
50 mM 0.0329 mL 0.1646 mL 0.3291 mL 0.8228 mL
60 mM 0.0274 mL 0.1371 mL 0.2743 mL 0.6856 mL
80 mM 0.0206 mL 0.1028 mL 0.2057 mL 0.5142 mL
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Chymostatin Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Chymostatin9076-44-2SARS-CoVVirus ProteaseNF-κBInterleukin RelatedSARS coronavirusNuclear factor-κBNuclear factor-kappaBILacute lung injuryanti-fungalpancreatitisInhibitorinhibitorinhibit

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