2. Metabolic Enzyme/Protease Apoptosis Cell Cycle/DNA Damage
  3. Phosphodiesterase (PDE) Apoptosis Caspase DNA/RNA Synthesis
  4. CD00509

CD00509 is a tyrosyl-DNA phosphodiesterase (PDE) Tdp1 inhibitor with an IC50 of 0.71 μM. CD00509 increases DNA damage and promotes apoptosis of MCF-7 cells. CD00509 can sensitize breast cancer cells and wild-type murine embryonic fibroblasts (MEFs) to Camptothecin (CPT) (HY-16560). CD00509 can be used for the study of cancers such as breast cancer.

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CD00509

CD00509 Chemical Structure

CAS No. : 27430-18-8

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Based on 1 publication(s) in Google Scholar

CD00509 Related Antibodies

Top Publications Citing Use of Products

View All Phosphodiesterase (PDE) Isoform Specific Products:

View All Isoforms
PDE1 PDE2 PDE3 PDE4 PDE5 PDE6 PDE7 PDE9 PDE10 PDE11 PDE12 PDE8 Autotaxin

View All Caspase Isoform Specific Products:

View All Isoforms
Caspase 1 Caspase 2 Caspase 3 Caspase 4 Caspase 5 Caspase 6 Caspase 7 Caspase 8 Caspase 9 Caspase 10 Caspase 12 Caspase Caspase 11 Caspase-13

  • Biological Activity

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Description

CD00509 is a tyrosyl-DNA phosphodiesterase (PDE) Tdp1 inhibitor with an IC50 of 0.71 μM. CD00509 increases DNA damage and promotes apoptosis of MCF-7 cells. CD00509 can sensitize breast cancer cells and wild-type murine embryonic fibroblasts (MEFs) to Camptothecin (CPT) (HY-16560). CD00509 can be used for the study of cancers such as breast cancer[1][2][3].

IC50 & Target[1]

PDEI

0.71 μM (IC50)

Caspase-3

 

Caspase-7

 

In Vitro

CD00509 (30 μM, 1-2 h) increases γ-H2AX Foci and DNA breaks in CPT-treated MCF-7 Cells[2].
CD00509 (10 μM, 0-96 h) reduces the number of MCF-7 cells, has almost no effect on normal breast epithelial cells, and significantly increases the caspase-3/7 activation of MCF-7 cells and co-treatment with CPT or Rucaparib (HY-10617A) is more lethal[2].
CD00509 (10 μM) effectively restores the attenuated TPI activity of PGP-inactivated MEFs to wildtype levels[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

CD00509 Related Antibodies
Molecular Weight

222.22

Formula

C9H6N2O3S
CAS No.
Appearance

Solid

Color

Light yellow to yellow

SMILES

S=C1NC(=O)C(C(N1)=O)=CC2=CC=CO2
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility
In Vitro: 

DMSO : 12.5 mg/mL (56.25 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 4.5000 mL 22.5002 mL 45.0005 mL
5 mM 0.9000 mL 4.5000 mL 9.0001 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

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V2

In Vivo Dissolution Calculator
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 4.5000 mL 22.5002 mL 45.0004 mL 112.5011 mL
5 mM 0.9000 mL 4.5000 mL 9.0001 mL 22.5002 mL
10 mM 0.4500 mL 2.2500 mL 4.5000 mL 11.2501 mL
15 mM 0.3000 mL 1.5000 mL 3.0000 mL 7.5001 mL
20 mM 0.2250 mL 1.1250 mL 2.2500 mL 5.6251 mL
25 mM 0.1800 mL 0.9000 mL 1.8000 mL 4.5000 mL
30 mM 0.1500 mL 0.7500 mL 1.5000 mL 3.7500 mL
40 mM 0.1125 mL 0.5625 mL 1.1250 mL 2.8125 mL
50 mM 0.0900 mL 0.4500 mL 0.9000 mL 2.2500 mL
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CD00509 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

CD0050927430-18-8CD 00509CD-00509Phosphodiesterase (PDE)ApoptosisCaspaseDNA/RNA SynthesisDNA repairMCF-7Camptothecinanti-cancerDNA damagebreast cancerInhibitorinhibitorinhibit

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