2. Apoptosis Anti-infection Immunology/Inflammation Metabolic Enzyme/Protease Protein Tyrosine Kinase/RTK Neuronal Signaling MAPK/ERK Pathway PI3K/Akt/mTOR
  3. Ferroptosis Parasite PGE synthase Lipoxygenase Trk Receptor p38 MAPK mTOR Akt
  4. Cannflavin B

Cannflavin B  (Synonyms: Canniflavone)

Cat. No.: HY-W749694 Purity: 98.36%
COA
SDS
Handling Instructions Technical Support

Cannflavin B is a flavonoid compound that can be isolated from Cannabis sativa L. Cannflavin B is inhibitors of PGE2 release (IC50: 0.7 μM), mPGES-1 (IC50: 3.7 μM), and 5-lipoxygenase. Cannflavin B has multiple activities such as anti-inflammatory, antioxidant, anti-glycation, anti-ferroptosis, anti-tumor, and anti-Leishmania (IC50: 14 μM). Cannflavin B can also inhibit the TrkB-BDNF signaling pathway.

For research use only. We do not sell to patients.

Cannflavin B Chemical Structure

Cannflavin B Chemical Structure

CAS No. : 76735-58-5

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Customer Review

Based on 1 publication(s) in Google Scholar

Cannflavin B Related Antibodies

Top Publications Citing Use of Products

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Amebae Coccidia Leishmania Mite Plasmodium Toxoplasma Trypanosoma Schistosome

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5-LOX 12-LOX 15-LOX

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TrkA TrkB TrkC Trk

View All p38 MAPK Isoform Specific Products:

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p38 MAPK p38α p38β MAPK13 p38δ p38γ

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mTOR mTORC1 mTORC2

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Akt Akt1 Akt2 Akt3

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Cannflavin B is a flavonoid compound that can be isolated from Cannabis sativa L. Cannflavin B is inhibitors of PGE2 release (IC50: 0.7 μM), mPGES-1 (IC50: 3.7 μM), and 5-lipoxygenase. Cannflavin B has multiple activities such as anti-inflammatory, antioxidant, anti-glycation, anti-ferroptosis, anti-tumor, and anti-Leishmania (IC50: 14 μM). Cannflavin B can also inhibit the TrkB-BDNF signaling pathway[1][2][3][4].

IC50 & Target[1][3]

5-LOX

 

TrkB

 

In Vitro

Cannflavin B (0-20 μM; 6 h) inhibits BDNF-induced Arc expression and phosphorylation of TrkB, Mapk, Akt, and mTor in primary mouse cortical neurons[1].
Cannflavin B (10 μM; 24 h) reduces BDNF-induced neurite number and length in Neuro2a cells stably expressing TrkB[1].
Cannflavin B (0-400 µM; 0.5-28 h) increases cell viability and inhibits ferroptosis and lipid peroxidation in Erastin (HY-15763)-treated HaCaT cells[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cannflavin B Related Antibodies

Western Blot Analysis[1]

Cell Line: Mouse primary cortical neurons
Concentration: 0, 1, 5, 10 and 20 μM
Incubation Time: 6 h
Result: Inhibited the levels of Arc in a dose-dependent manner.

Cell Viability Assay[2]

Cell Line: Erastin (HY-15763)-treated HaCaT cells
Concentration: 1.25, 2.5, 5 , 10 and 20 µM
Incubation Time: Pretreated with 4 h, then co-incubation for 24 h
Result: Significantly restored cell viability.
Molecular Weight

368.38

Formula

C21H20O6
CAS No.
Appearance

Solid

Color

Light yellow to yellow

SMILES

O=C1C=C(C2=CC(OC)=C(O)C=C2)OC3=CC(O)=C(C/C=C(C)\C)C(O)=C13
Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

-20°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility
In Vitro: 

DMSO : 50 mg/mL (135.73 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.7146 mL 13.5729 mL 27.1459 mL
5 mM 0.5429 mL 2.7146 mL 5.4292 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
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Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 1.25 mg/mL (3.39 mM); Clear solution

    This protocol yields a clear solution of ≥ 1.25 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (12.5 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: 98.36%

SDS (251 KB)

COA (247 KB) HNMR (153 KB) RP-HPLC (251 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.7146 mL 13.5729 mL 27.1459 mL 67.8647 mL
5 mM 0.5429 mL 2.7146 mL 5.4292 mL 13.5729 mL
10 mM 0.2715 mL 1.3573 mL 2.7146 mL 6.7865 mL
15 mM 0.1810 mL 0.9049 mL 1.8097 mL 4.5243 mL
20 mM 0.1357 mL 0.6786 mL 1.3573 mL 3.3932 mL
25 mM 0.1086 mL 0.5429 mL 1.0858 mL 2.7146 mL
30 mM 0.0905 mL 0.4524 mL 0.9049 mL 2.2622 mL
40 mM 0.0679 mL 0.3393 mL 0.6786 mL 1.6966 mL
50 mM 0.0543 mL 0.2715 mL 0.5429 mL 1.3573 mL
60 mM 0.0452 mL 0.2262 mL 0.4524 mL 1.1311 mL
80 mM 0.0339 mL 0.1697 mL 0.3393 mL 0.8483 mL
100 mM 0.0271 mL 0.1357 mL 0.2715 mL 0.6786 mL
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Cannflavin B Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Cannflavin B76735-58-5CanniflavoneFerroptosisParasitePGE synthaseLipoxygenaseTrk Receptorp38 MAPKmTORAktProstaglandin E synthaseLOXTropomyosin related kinase receptor Mammalian target of RapamycinPKBProtein kinase Bcortical neuronsNeuro2a cellsHaCaT cellsInhibitorinhibitorinhibit

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