2. Cell Cycle/DNA Damage
  3. Topoisomerase
  4. BNS-22

BNS-22 is a DNA topoisomerase II (TOP2) catalytic inhibitor with the IC50 values of 2.8 μM and 0.42 μM for human TOP2α and TOP2β, respectively. BNS-22 induces abnormal division and has anti-proliferative activity.

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BNS-22 Chemical Structure

BNS-22 Chemical Structure

CAS No. : 1151668-24-4

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Based on 1 publication(s) in Google Scholar

BNS-22 Related Antibodies

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Description

BNS-22 is a DNA topoisomerase II (TOP2) catalytic inhibitor with the IC50 values of 2.8 μM and 0.42 μM for human TOP2α and TOP2β, respectively. BNS-22 induces abnormal division and has anti-proliferative activity[1].
IC50 & Target[1]

topoisomerase II alpha

2.8 μM (IC50, in human)

topoisomerase II beta

0.42 μM (IC50, in human)

In Vitro

BNS-22 (0-30 μM, 24-48 h) can inhibit cell growth and influence on cell cycle progression of the human cervical epidermoid carcinoma cell line HeLa in a dose-dependent manner[1].
BNS-22 (0-30 μM, 0-6 h) does not induce DNA damage and has an antagonistic effect on TOP2 toxin-mediated DNA damage[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

BNS-22 Related Antibodies

Cell Proliferation Assay[1]

Cell Line: HeLa cell lines
Concentration: 0-30 μM
Incubation Time: 24 h, 48 h
Result: Inhibited cell growth with the IC50 value of 4.9 μM after 24 hours and 1.0 μM after 48 hours.

Cell Cycle Analysis[1]

Cell Line: HeLa cell lines
Concentration: 0-30 μM
Incubation Time: 24 h
Result: Increased number of cells in G2/M phase.
Disrupted mitotic spindle formation and induced polyploid cell formation at 3 μM.

Western Blot Analysis[1]

Cell Line: HeLa cell lines
Concentration: 0-30 μM
Incubation Time: 0, 30 min, 1 h, 2 h, 4 h, 6 h
Result: Decreased etoposide-induced γ-H2AX expression and decreased total cellular TOP2β levels, but not TOP2α levels.
Molecular Weight

407.46

Formula

C24H25NO5
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

O=C1C=C(CCC)C2=C(OC)C=C(OC)C(C(N3CCCC4=C3C=CC=C4)=O)=C2O1
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 50 mg/mL (122.71 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.4542 mL 12.2711 mL 24.5423 mL
5 mM 0.4908 mL 2.4542 mL 4.9085 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

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In Vivo Dissolution Calculator
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Working solution concentration: mg/mL
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.4542 mL 12.2711 mL 24.5423 mL 61.3557 mL
5 mM 0.4908 mL 2.4542 mL 4.9085 mL 12.2711 mL
10 mM 0.2454 mL 1.2271 mL 2.4542 mL 6.1356 mL
15 mM 0.1636 mL 0.8181 mL 1.6362 mL 4.0904 mL
20 mM 0.1227 mL 0.6136 mL 1.2271 mL 3.0678 mL
25 mM 0.0982 mL 0.4908 mL 0.9817 mL 2.4542 mL
30 mM 0.0818 mL 0.4090 mL 0.8181 mL 2.0452 mL
40 mM 0.0614 mL 0.3068 mL 0.6136 mL 1.5339 mL
50 mM 0.0491 mL 0.2454 mL 0.4908 mL 1.2271 mL
60 mM 0.0409 mL 0.2045 mL 0.4090 mL 1.0226 mL
80 mM 0.0307 mL 0.1534 mL 0.3068 mL 0.7669 mL
100 mM 0.0245 mL 0.1227 mL 0.2454 mL 0.6136 mL
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BNS-22 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

BNS-221151668-24-4BNS22BNS 22TopoisomeraseTOP2 catalytic inhibitorDNA topoisomerase IITOP2αTOP2βabnormal divisioncancerHeLaInhibitorinhibitorinhibit

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