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  6. Baptifoline

Baptifoline is an alkaloid isolated from the rhizome of Caulophyllum thalictroides.

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Baptifoline Chemical Structure

Baptifoline Chemical Structure

CAS No. : 732-50-3

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Description

Baptifoline is an alkaloid isolated from the rhizome of Caulophyllum thalictroides[1].

Molecular Weight

260.33

Formula

C15H20N2O2
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

O=C1N2C([C@@]3([H])CN(CC[C@@H](O)C4)[C@@]4([H])[C@]([H])(C3)C2)=CC=C1
Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

Ethanol : ≥ 50 mg/mL (192.06 mM)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.8413 mL 19.2064 mL 38.4128 mL
5 mM 0.7683 mL 3.8413 mL 7.6826 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% EtOH    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 1.25 mg/mL (4.80 mM); Clear solution

    This protocol yields a clear solution of ≥ 1.25 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL EtOH stock solution (12.5 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% EtOH    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 1.25 mg/mL (4.80 mM); Clear solution

    This protocol yields a clear solution of ≥ 1.25 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL EtOH stock solution (12.5 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation

Purity: ≥99.0%

SDS (251 KB)

COA (250 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
Ethanol 1 mM 3.8413 mL 19.2064 mL 38.4128 mL 96.0320 mL
5 mM 0.7683 mL 3.8413 mL 7.6826 mL 19.2064 mL
10 mM 0.3841 mL 1.9206 mL 3.8413 mL 9.6032 mL
15 mM 0.2561 mL 1.2804 mL 2.5609 mL 6.4021 mL
20 mM 0.1921 mL 0.9603 mL 1.9206 mL 4.8016 mL
25 mM 0.1537 mL 0.7683 mL 1.5365 mL 3.8413 mL
30 mM 0.1280 mL 0.6402 mL 1.2804 mL 3.2011 mL
40 mM 0.0960 mL 0.4802 mL 0.9603 mL 2.4008 mL
50 mM 0.0768 mL 0.3841 mL 0.7683 mL 1.9206 mL
60 mM 0.0640 mL 0.3201 mL 0.6402 mL 1.6005 mL
80 mM 0.0480 mL 0.2401 mL 0.4802 mL 1.2004 mL
100 mM 0.0384 mL 0.1921 mL 0.3841 mL 0.9603 mL
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Baptifoline Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Baptifoline732-50-3OthersalkaloidCaulophyllum thalictroidesInhibitorinhibitorinhibit

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