2. Apoptosis
  3. IAP Apoptosis
  4. AZD5582 dihydrochloride

AZD5582 dihydrochloride is an antagonist of the inhibitor of apoptosis proteins (IAPs), which binds to the BIR3 domains cIAP1, cIAP2, and XIAP with IC50s of 15, 21, and 15 nM, respectively. AZD5582 induces apoptosis.

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AZD5582 dihydrochloride Chemical Structure

AZD5582 dihydrochloride Chemical Structure

CAS No. : 1883545-51-4

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10 mM * 1 mL in DMSO In-stock
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Customer Review

Based on 12 publication(s) in Google Scholar

Other Forms of AZD5582 dihydrochloride:

AZD5582 dihydrochloride Related Antibodies

Top Publications Citing Use of Products

    AZD5582 dihydrochloride purchased from MedChemExpress. Usage Cited in: Cancer Immunol Res. 2023 Feb 8;CIR-22-0494.  [Abstract]

    AZD5582 (0.1-1 μM; 24 h) decreases the expression of cIAP1, cIAP2 and XIAP in GM-CSF DCs.

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    cIAP cIAP-1 cIAP-2 XIAP

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    Description

    AZD5582 dihydrochloride is an antagonist of the inhibitor of apoptosis proteins (IAPs), which binds to the BIR3 domains cIAP1, cIAP2, and XIAP with IC50s of 15, 21, and 15 nM, respectively. AZD5582 induces apoptosis[1].

    IC50 & Target[1]

    cIAP1

    15 nM (IC50)

    cIAP2

    21 nM (IC50)

    XIAP

    15 nM (IC50)

    In Vitro

    AZD5582 (20 nM; 48 hours) inhibits cell viability by cooperation with IFNγ or viral double-stranded RNA (dsRNA) in H1975 NSCLC cells[2].
    AZD5582 (20 nM; 17 or 25 hours) downregulates cIAP-1, activates RIPK1 (upstream regulator of caspase-8), and triggers the activation of extrinsic (caspase-8) and intrinsic (caspase-9) apoptosis pathways, causing the cleavage of caspase-3 and caspase-7[2].
    AZD5582 (20 nM; 48 hours) involves in apoptosis due to induction of cell death and active caspase-3/8 activities by AZD5582 and IFNγ co-treatment in HCC827 NSCLC cells[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    AZD5582 dihydrochloride Related Antibodies

    Cell Viability Assay[2]

    Cell Line: H1975 NSCLC cell line
    Concentration: 20 nM
    Incubation Time: 48 hours
    Result: Cooperated with IFNγ or viral double-stranded RNA (dsRNA) to inhibit cell viability even cell death.

    Apoptosis Analysis[2]

    Cell Line: HCC827 NSCLC cell line
    Concentration: 20 nM
    Incubation Time: 48 hours
    Result: Had an inhibitory effect on cell viability by cooperating with IFNγ.

    Western Blot Analysis[2]

    Cell Line: H1975 NSCLC cell line
    Concentration: 20 nM
    Incubation Time: 17 or 25 hours
    Result: Down-regulated cIAP-1, activated RIPK1 (upstream regulator of caspase-8), triggered the cleavage (activation) of caspase-3,7,8 and 9.
    In Vivo

    AZD5582 (intravenous injection; 0.1-3.0 mg/kg; once a week; 2 weeks) causes degradation of cIAP1 and caspase 3 cleavage in tumor cells, and after a two-week treatment, the tumors largely resolved; when the mice are given a medium dose (0.5 mg/kg) of AZD5582, cIAP1 degrades after administration, but it takes a while time to reach apoptosis-inducing effect[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: MDA-MB-231 xenograft-bearing mice[1]
    Dosage: 0.1 mg/kg, 0.5 mg/kg, 3.0 mg/kg
    Administration: Intravenous injection; once a week; 2 weeks
    Result: Resulted in cIAP1 degradation and caspase-3 cleavage within tumor cells and causes substantial tumor regressions following two weekly doses of 3.0 mg/kg
    Molecular Weight

    1088.21

    Formula

    C58H80Cl2N8O8
    CAS No.
    Appearance

    Solid

    Color

    Off-white to light yellow

    SMILES

    CN[C@@H](C)C(N[C@H](C(N1[C@H](C(N[C@H]2C3=CC=CC=C3C[C@H]2OCC#CC#CCO[C@H]4[C@@H](NC([C@@H]5CCCN5C([C@@H](NC([C@H](C)NC)=O)C6CCCCC6)=O)=O)C(C=CC=C7)=C7C4)=O)CCC1)=O)C8CCCCC8)=O.Cl.Cl
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    4°C, protect from light

    *In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

    Purity & Documentation
    References
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    AZD5582 dihydrochloride Related Classifications

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    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    AZD55821883545-51-4AZD 5582AZD-5582IAPApoptosisInhibitorinhibitorinhibit

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