YTHDF1 Antibody (YA1429)

YTHDF1 Antibody (YA1429) is a rabbit-derived non-conjugated IgG antibody (Clone NO.: YA1429), targeting YTHDF1, with a predicted molecular weight of 61 kDa (observed band size: 61 kDa). YTHDF1 Antibody (YA1429) can be used for WB, IP experiment in human, hamster background.

YTHDF1 is a protein that specifically recognizes and binds to N6-methyladenosine (m6A)-containing mRNAs, regulating their stability and thus influencing gene expression. m6A is a modification found at internal sites of mRNAs and some non-coding RNAs, playing a role in mRNA stability and processing. YTHDF1 promotes the degradation of m6A-containing mRNAs by interacting with the CCR4-NOT complex (By similarity). The YTHDF paralogs (YTHDF1, YTHDF2, and YTHDF3) share m6A-containing mRNA targets and function redundantly in mediating mRNA degradation and cellular differentiation. In the hippocampus, YTHDF1 facilitates learning and memory formation by binding to m6A-modified neuronal mRNAs. It also regulates axon guidance through interaction with m6A-containing ROBO3 transcripts. In myeloid dendritic cells, YTHDF1 acts as a negative regulator of antigen cross-presentation, limiting anti-tumor responses by reducing tumor-antigen cross-presentation efficiency during tumorigenesis. Additionally, YTHDF1 undergoes liquid-liquid phase separation upon binding to mRNAs with multiple m6A-modified residues, promoting the formation of membraneless compartments such as P-bodies or stress granules (By similarity). These mRNA-YTHDF complexes then partition into various endogenous membraneless compartments.

P59326

Predicted band size: 61 kDa; Observed band size: 72 kDa

Affinity Purified

Non-conjugated

Unmodified

AB_3103792

Epigenetics and Nuclear Signaling

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human, Hamster

WB: 1/500-1/1000;IP: 1/20

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  Western blot analysis of extracts from HepG2(lane 2(20ug) , Hela(lane 3(20ug) and NIH/3T3(lane 4(20ug) using YTHDF1 Antibody (HY-P81684) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

WB, IP

Liquid

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

Recombinant protein of mouse YTHDF1