WDR77 Antibody (YA5281)

WDR77 Antibody (YA5281) is a mouse-derived and non-conjugated monoclonal antibody, targeting to WDR77. It can be applicated for WB, ICC assays, in the background of human, mouse, rat.

WDR77 functions as a non-catalytic component within the methylosome complex, alongside PRMT5 and CLNS1A, orchestrating the modification of specific arginines to dimethylarginines in spliceosomal Sm proteins and histones. This modification directs Sm proteins to the survival of motor neurons (SMN) complex, facilitating their assembly into small nuclear ribonucleoprotein core particles. Beyond its role in RNA processing, WDR77 may play a part in transcription regulation. The methylosome complex extends its methylating activity to Piwi proteins (PIWIL1, PIWIL2, and PIWIL4), essential for their interaction with Tudor domain-containing proteins and subsequent localization to the meiotic nuage. WDR77 engages in a complex interplay with other components such as PRMT1, ERH, and CLNS1A, with specific interactions observed with PRMT5, various Sm proteins, SUZ12, histone H2A, CTDP1, LSM11, APEX1, AR, NKX3-1, CHTOP, FAM47E, and TSC22D2. Its intricate network of interactions underscores WDR77's multifaceted involvement in epigenetic modifications and regulatory pathways.

Q9BQA1

Observed band size: 42 kDa

affinity chromatography.

Non-conjugated

Unmodified

Primary Antibody; Mouse Monoclonal Antibody

Monoclonal

Mouse

Human, Mouse, Rat

WB: 1:1000; ICC: 1:100

WB, ICC

Liquid

Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

Purified recombinant human WDR77 protein fragments expressed in E.coli.